Abstract
The productivity of strains isolated in a screening program is ordinarily very low; commonly a few milligrams of metabolite per liter of culture. Initial evaluation of the biological potential of a new metabolite and its chemical characterization requires grams of pure material. Kilograms and tens of kilograms are then needed for animal toxicology studies and later for clinical trials. Therefore, from the initial stages of the development of a new metabolite it is necessary to improve the productivity of the fermentation process. In later stages of development the increase of yields is of paramount importance to define an industrial process by which the substance can be produced at an acceptable cost.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baltz, R. H., 1986, Mutagenesis in Streptomyces spp., in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 184–190, American Society for Microbiology, Washington, D.C.
Calam, C. T., 1986, Physiology of the overproduction of secondary metabolites, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 27–50, Butterworths, London.
Chater, K. F., 1990, The improving prospects for yield increase by genetic engineering in antibiotic-producing streptomycetes, Bio/Technology 8: 115.
Elender, R. P., 1987, Microbial screening, selection and strain improvement, in Basic Biotechnology ( J. Bu’Lock and B. Kristiansen, eds.), pp. 217–251, Academic Press, New York.
Elander, R. P., and Vournakis, J. N., 1986, Genetic aspects of overproduction of antibiotics and other secondary metabolites, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 63–80, Butterworths, London.
Holt, G., Saunders, G., and Dales, R., 1986, Genetic approaches to overproduction of fungal antibiotics, in Overproduction of Microbial Metabolites. Strain Improvement and Process Control Strategies ( Z. Vanék and Z. Hostâlek, eds.), pp. 81–104, Butterworths, London.
Katz, L., and Hutchinson, C. R., 1992, Genetic engineering of antibiotic producing organisms, Annu. Rep. Med. Chem. 27: 129.
Matsushima, P., and Baltz, R. H., 1986, Protoplast fusion, in Manual of Industrial Microbiology and Biotechnology (A. L. Demain and N. A. Solomon, eds.), pp. 170183, American Society for Microbiology, Washington, D.C.
Nisbet, L. J.,and Winstanley, D. J.,(eds.), 1983, Bioactive Products 2. Development and Production,Academic Press, New York.
Normansell, I. D., 1986, Isolation of Streptomyces mutants improved for antibiotic production, in The Bacteria, Vol. IX ( S. W. Queener and L. E. Day, eds.), pp. 95118, Academic Press, New York.
Queener, S. W., and Lively, D. H., 1986, Screening and selection for strain improvement, in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 155–169, American Society for Microbiology, Washington, D.C.
Rowlands, R. T., 1992, Strain improvement and strain stability, in Biotechnology of Filamentous Fungi ( B. S. Finkelstein and C. Ball, eds.), pp. 41–64, Butterworths, London.
Saunders, V. A., and Saunders, J. R., 1987, Microbial Genetics Applied to Biotechnology. Principles and Techniques of Gene Transfer and Manipulation, Croom Helm, London.
Skatrud, P. L., Tietz, A. J., Ingolia, T. D., Cantwell, C. A., Fisher, D. L., Chapmann, J. L., and Queener, S. W., 1989, Use of recombinant DNA to improve production of cephalosporin C by Cephalosporium acremonium, Bio/Technology 7: 477.
Stuttzman-Engwall, K. J., Otten, S. L., and Hutchinson, C. R., 1992, Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius, J. Bacteriol. 174: 144.
Trilli, A., 1986, Scale-up of fermentations, in Manual of Industrial Microbiology and Biotechnology ( A. L. Demain and N. A. Solomon, eds.), pp. 277–307, American Society for Microbiology, Washington, D.C.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lancini, G., Lorenzetti, R. (1993). Strain Improvement and Process Development. In: Biotechnology of Antibiotics and Other Bioactive Microbial Metabolites. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9522-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9522-6_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9524-0
Online ISBN: 978-1-4757-9522-6
eBook Packages: Springer Book Archive