Abstract
The quenching of the fluorescence of biomacromolecules by solute quenchers has become a widely used and powerful technique (Lehrer, 1976; Lehrer and Leavis, 1989; Lakowicz, 1983; Eftink and Ghiron, 1981; Eftink, 1991). Such quenching reactions have been used primarily to obtain topographical information about proteins, nucleic acids, and membrane systems. The accessibility of intrinsic or extrinsic fluorescence probes (e.g., the amino acid, tryptophan), which are attached to a biomacromolecule, to small quenchers (e.g., iodide, acrylamide, oxygen) is directly determined by such reactions. Conformational changes in the biomacromolecule can then be monitored in terms of changes in the accessibility to the quencher of the fluorophore. In addition to such topographical information, in some cases information about the conformational dynamics of globular proteins has been obtained with solute quenching reactions.
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References
Acuna, A. U., Lopez-Hernandez, F. J., and Oton, J. M., 1982, Biophys. Chem. 16:253–260.
Altekar, W., 1977, Biopolymers 16:341–368.
Alwattar, A. H., Lumb, M. D., and Birks, J. B., 1973, in Organic Molecular Photophysics, Vol. 1 (J. B. Birks, ed.), Wiley-Interscience, New York, pp. 403–454.
Ando, T., and Asai, H., 1980, J. Biochem. 88:255–264.
Ando, T., Fujisaki, H., and Asai, H., 1980, J. Biochem. 88:265–276.
Andre, J. C., Niclause, M., and Ware, W. R., 1978, Chem. Phys. 28:371–377.
Badea, M. G., and Brand, L., 1979, Methods Enzymol. 61:378–425.
Batenburg, A. M., Bougis, P. E., Rochat, H., Verkleij, A. J., and deKruijff, B., 1985, Biochemistry 24:7101–7110.
Beechem, J. R., and Brand, L., 1985, Annu. Rev. Biochem. 54:43–71.
Beechem, J. R., Ameloot, M., Knutson, J. R., and Brand, L., 1991, in Fluorescence Spectroscopy: Theory and Applications, Vol. 1 (J. R. Lakowicz, ed.), Plenum Press, New York.
Bieri, V. G., and Wallach, D. F. H., 1975, Biochim. Biophys. Acta 406:415–423.
Blackwell, M. F., Gounairs, K., Zara, S. J., and Barber, J., 1987, Biophys. J. 51:735–744.
Blatt, E., and Sawyer, W. H., 1985, Biochim. Biophys. Acta 822:43–62.
Blatt, E., Chatelier, C., and Sawyer, W. H., 1986, Biophys. J. 50:349–356.
Bohorquez, M. del V., Cosa, J. J., Garcia, N. A., and Previtali, C. M., 1984, Photochem. Photobiol 40:201–205.
Bushueva, T. L., Busel, E. P., and Burstein, E. A., 1975, Stud. Biophys. 52:41–52.
Calhoun, D. B., Vanderkooi, J. M., and Englander, S. W., 1983, Biochemistry 22:1533–1539.
Cavatorta, P., Favilla, R., and Mazzini, A., 1979, Biochim. Biophys. Acta 578:541–546.
Chalpin, D. B., and Kleinfeld, A. M., 1983, Biochim. Biophys. Acta 731:465–474.
Chattopadhayay, A., and London, E., 1987, Biochemistry 26:39–45.
Chen, L. X. Q., Longworth, J. W., and Fleming, G. R., 1987, Biophys. J. 51:685–873.
Chen, R. F., 1973, Arch. Biochem. Biophys. 158:605–622.
Danileviciute, M., Adomeniene, O., and Dienys, G., 1981, Org. React. (USSR) 18:217–224.
Demmer, D. R., James, D. R., Steer, D. P., and Verrall, R. E., 1987, Biochemistry 20:4369–4377.
DeWolf, M. J. S., Van Dessel, G. A. F., Largrou, A. R., Hilderson, H. J. J., and Dierick, W. S. H., 1987, Biochemistry 26:3799–3806.
Dowd, J. E., and Riggs, D. S., 1965, J. Biol. Chem. 240:863–869.
Edwards, A. M. M, and Silva, E. S., 1986, Radiat. Environ. Biophys. 25:113–122.
Eftink, M. R., 1991, in Fluorescence Spectroscopy, Vol. II, (J. R. Lakowicz, ed.), Plenum Press, New York (in press).
Eftink, M. R., and Ghiron, C. A., 1976a, J. Phys. Chem. 80:486–493.
Eftink, M. R., and Ghiron, C. A., 1976b, Biochemistry 15:672–680.
Eftink, M. R., and Ghiron, C. A., 1978, Biochemistry 7: 5546–5551.
Eftink, M. R., and Ghiron, C. A., 1981, Anal. Biochem. 114:199–227.
Eftink, M. R., and Ghiron, C. A., 1984, Biochemistry 23:3891–3899.
Eftink, M. R., and Ghiron, C. A., 1987a, Photochem. Photobiol. 45:745–748.
Eftink, M. R., and Ghiron, C. A., 1987b, Biochim. Biophys. Acta 916:343–349.
Eftink, M. R., and Hagaman, K. A., 1987, Biophys. Chem. 26:277–282.
Eftink, M. R., and Selvidge, L. A., 1982, Biochemistry 21:117–125.
Eftink, M. R., and Wasylewski, Z., 1989, Biochemistry 28:382–391.
Eftink, M. R., and Wasylewski, Z., 1988b, Biophys. Chem. 32:121–130.
Eftink, M. R., Zajicek, J. L., and Ghiron, C. A., 1977, Biochim. Biophys. Acta 491:473–481.
Eftink, M. R., Selva, T. J., and Wasylewski, Z., 1987, Photochem. Photobiol. 46:23–30.
Fato, R., Battino, M. D., Esposit, G. P., Castelli, G. P., and Lenaz, G., 1986, Biochemistry 25:3378–3390.
Ferguson, B. Q., and Yang, D. C. H., 1986, Biochemistry 25:529–539.
Froehlich, P. M., and Nelson, K., 1978, J. Phys. Chem. 82:2401–2403.
Geisthardt, D., and Kruppa, J., 1987, Anal. Biochem. 160:184–191.
Georghiou, S., Thompson, M., and Mukhopadhyay, A. K., 1982, Biochim. Biophys. Acta 688:441–452.
Ghiron, C. A., Eftink, M. R., Porter, M. A., and Hartman, F. C., 1988, Arch. Biochem. Biophys. 260:267–272.
Gratton, E., Alpert, B., Jameson, D. M., and Weber, G., 1984, Biophys. J. 45:789–794.
Gomez-Fernandez, J. C., Baena, M. D., Teruel, J. A., Villalain, J., and Vidal, C. J., 1985, J. Biol. Chem. 260:7168–7170.
Hansen, D., Altschmied, L., and Hillen, W., 1987, J Biol. Chem. 262:14030–14035.
Hashimoto, K., and Albridge, W. N., 1970, Biochem. Pharmacol. 19:2591–2603.
Havel, H. A., Kauffman, E. W., and Elzinga, P. N., 1988, Biochim. Biophys. Acta 955:154–163.
Hill, B. C., Horowitz, P. M., and Robinson, N. C., 1986, Biochemistry 25:2287–2292.
Johnson, D. A., and Yguerabide, J., 1985, Biophys. J. 48:949–955.
Kauffman, R. F., Chapman, C. J., and Pfeiffer, D. R., 1983, Biochemistry 22:3985–3992.
Kan, J. H., Wijnaendts van Resandt, R. W., and Dekkens, H. P. J. M., 1986, J. Biomol. Struct. Dyn. 3:827–842.
Knutson, J. R., Baker, S. H., Cappucino, A. G., Walbridge, D. W., and Brand, L., 1983a, Photochem. Photobiol. 37:S21.
Knutson, J. R., Beechem, J. M., and Brand, L., 1983b, Chem. Phys. Lett. 102:501–507.
Lakowicz, J. R., 1983, Principles of Fluorescence Spectroscopy, Plenum Press, New York, Chapter 9.
Lakowicz, J. R., Hogen, D., and Omann, G., 1977, Biochim. Biophys. Acta 471:401–411.
Lakowicz, J. R., and Weber, G., 1973a, Biochemistry 12:4161–4170.
Lakowicz, J. R., and Weber, G., 1973b, Biochemistry 12:4171–4179.
Lakowicz, J. R., Johnson, M. L., Joshi, N., Gryczynski, I., and Laczko, G., 1986, Chem. Phys. Lett. 131:343–348.
Lakowicz, J. R., Joshi, N. B., Johnson, M. L., Szmacinski, H., and Gryczynski, I., 1987, J. Biol. Chem. 262:10907–10910.
Lee, H.-J., Chen, Y.-H., and Chang, G.-G., 1988, Biochim. Biophys. Acta 955:119–127.
Lehrer, S. S., 1971, Biochemistry 10:3254–3263.
Lehrer, S. S., 1976, in Biochemical Fluorescence: Concepts, Vol. 2 (R. Chen and H. Edelhoch, eds.), Dekker, New York, pp. 515–544.
Lehrer, S. S., and Leavis, P. C., 1978, Methods Enzymol. 49:222–236.
London, E., and Feigenson, G. W., 1981, Biochemistry 20:1932–1938.
Markello, T., Zlotnick, A., Everett, J., Tennyson, J., and Holloway, P. W., 1985, Biochemistry 24:2895–2901.
Midoux, P., Wahl, P., Auchet, J.-C., and Monsigng, M., 1984, Biochim. Biophys. Acta 801:16–25.
Narasimhulu, S., 1988, Biochemistry 27:1147–1153.
Nemzek, T. L., and Ware, W. R., 1975, J. Chem. Phys. 62:477–489.
Olea, A. F., and Thomas, J. K., 1988, J. Am. Chem. Soc. 110:4494–4502.
O’Neil, K. T., Wolfe, H. R., Jr., Erickson-Viitanen, S., and DeGrado, W. F., 1987, Science 236:1454–1456.
Phillips, A. V., Robbins, D. J., Coleman, M. S., and Barkley, M. D., 1987, Biochemistry 26:2893–2903.
Pownall, H. J., and Smith, L. C., 1974, Biochemistry 13:2594–2597.
Ricci, R. W., and Kilichowski, K. B., 1974, J. Phys. Chem. 78:1953–1956.
Robbins, D. J., Deibel, M. R., Jr., and Markley, M. D., 1985, Biochemistry 24:7250–7257.
Ross, J. B. A., Schmidt, C., and Brand, L., 1981, Biochemistry 20:4369–4377.
Shinitzky, M., 1972, J. Chem. Phys. 56:5979–5981.
Shinitzky, M., and Rivnay, B., 1977, Biochemistry 16:982–986.
Sluyterman, L. A., and DeGraaf, M. J. M., 1970, Biochim. Biophys. Acta 200:595–597.
Sommers, P. B., and Kronman, M. J., 1980, Biophys. Chem. 11:217–232.
Steiner, R. F., and Kirby, E. P., 1969, J. Phys. Chem. 73:4130–4135.
Stryjewski, W., and Wasylewski, Z., 1986, Fur. J. Biochem. 158:547–553.
Stubbs, C. D., and Williams, B. W., 1991, in Fluorescence Spectroscopy, Vol. II (J. R. Lakowicz, ed.), Plenum Press, New York (in press).
Thulborn, K. R., and Sawyer, W. H., 1978, Biochim. Biophys. Acta 511:125–140.
Torgerson, P. M., 1984, Biochemistry 23:3002–3007.
Toulmé, J.-J., LeDuan, T., and Helene, C., 1984, Biochemistry 23:1195–1201.
Tyson, P. A., and Steinberg, M., 1987, J. Biol. Chem. 262:14030–14035.
Vaughn, W. M., and Weber, G., 1970, Biochemistry 9:464–473.
Wasylewski, Z., Koloczek, H., and Wasniowska, A., 1988, Eur. J. Biochem. 172:719–724.
Zinger, D., and Geacintov, N. E., 1988, Photochem. Photobiol. 47:181–188.
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Eftink, M.R. (1991). Fluorescence Quenching Reactions. In: Dewey, T.G. (eds) Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9513-4_1
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