A Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in Vitro

  • L. Devine
  • S. Lightman
  • J. Greenwood
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)


The traffic of lymphocytes into the neuroretina of the eye is an important aspect of the development of immune mediated diseases of this part of the central nervous system (CNS). At the interface between the systemic immune system and the parenchyma lies a blood-tissue barrier which in the eye is referred to as the blood-retinal barrier. This barrier is situated at two separate sites, one being composed of the vascular endothelium and the other of retinal pigment epithelium (RPE). The differential migration of lymphocytes across these two cellular barriers and the factors controlling this process are just beginning to be understood. We have shown that T cell line lymphocytes are capable of migrating through monolayers of both vascular endothelial and RPE monolayers in vitro. The principle adhesion molecules involved in this process being the LFA-1/ICAM-1 receptor-ligand pairing with VLA-4/VCAM-1 playing a role following cytokine activation of the monolayers.


Retinal Pigment Epithelium Experimental Autoimmune Encephalomyelitis Retinal Pigment Epithelium Cell Experimental Allergic Encephalomyelitis Purify Protein Derivative 
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Le trafic des lymphocytes dans la neurorétine de l’oeil est un aspect important du développement des maladies du système nerveux central (CNS) impliquant le système immunitaire. A l’interface entre le système immunitaire (circulation sanguine systémique) et le parenchyme se trouve une barrière sang-tissu qui au niveau de l’oeil porte le nom de barrière hémato-rétinienne. Cette barrière sépare l’endothélium vasculaire, d’une part et l’epithélium pigmenté de la rétine, d’autre part. Les différences de migration des lymphocytes à travers ces deux barrières cellulaires, ainsi que des facteurs contrôlant ce processus commencent tout juste à être étudiés. In vitro, nous avons montré que des lignées lymphocytaires T sont capables de migrer à travers les monocouches de l’endothélium vasculaire et de l’epithélium pigmenté de la rétine. Les principales molécules d’adhésion impliquées dans ce processus au niveau de ces monocouches sont les complexes récepteur-ligand LFAI/ICAM-1 et VLA-4/VCAM-1 après activation cytokines dépendante.


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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • L. Devine
    • 1
  • S. Lightman
    • 1
  • J. Greenwood
    • 1
  1. 1.Department of Clinical Ophthalmology, Institute of OphthalmologyUniversity College LondonLondonUK

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