Summary
The conditioned media of subconfluent and confluent monolayers of porcine astrocytes and different cell lines were used to reinduce in primary cultured endothelial cells of porcine brain microvessels in vivo properties like high activities of the marker enzymes alkaline phosphatase (ALP) and γ-glutamyltranspeptidase (γ-GT). After 7 days in culture, the activity of ALP increased only by incubation of cerebral endothelial cells with conditioned media from confluent adult porcine astrocytes and CHO-cells (fibroblasts), whereas the activity of γ-GT was significantly increased by conditioned media of astrocytes, CHOcells, chondrocytes and adenocarcinoma cells. However, the increase in γ-GT seems to be the result of an increase in the number of cells on the top of the monolayer with high γ-GT activities as revealed by histochemistry. The mechanism of the inductive effect on γ-GT seems to be not astrocyte specific and has to be clarified.
Résumé
Des milieux de culture conditionnés par des monocouches confluentes et subconfluentes d'astrocytes de porc et de differentes lignées cellulaires ont été utilisées pour réinduire, dans des cultures primaires de microvaisseaux de porc, des propriétés de la barrière hémato-encéphalique in vivo telles les fortes activités enzymatiques de la phosphatase alcaline (PA) ou de la γ-glutamytranspeptidase (γ-GT). Après sept jours de culture, l’activité de la PA a pu être réinduite seulement par les milieux conditionnés par les cultures confluentes d’astrocytes de porc adulte et d’une lignée de fibroblastes (CHO). Une augmentation de l’activité de la γ-GT a pu, dans les mêmes conditions, être observée avec des milieux conditionnés d’astrocytes, de cellules CHO, de chondrocytes et de cellules d’adénocarcinome. Toutefois, l’augmentation de l’activité de la γ-GT semble être le resultat de la multiplication au dessus de la monocouche de cellules possédant une forte activité γ-GT, comme le montrent des expériences d’histochimie. L’effet inducteur des milieux de cultures sur l’activité de la γ-GT ne semble pas spécifique des astrocytes et reste à élucider.
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Ahlemeyer, B., Matys, S., Brust, P. (1996). The Effect of Conditioned Medium of Porcine Astrocytes and Different Cell Lines on the Activity of the Marker Enzymes Alp and γ-GT in Endothelial Cells of Porcine Brain Microvessels. In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_38
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DOI: https://doi.org/10.1007/978-1-4757-9489-2_38
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