The Blood-CSF Barrier in Vitro

  • Ursula Gath
  • Ansgar Hakvoort
  • H.-J. Galla
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)

Summary

We established an in vitro-model for the blood-CSF barrier by culturing epithelial cells from the porcine choroid plexus. The cells express their epithelial properties in culture and form confluent monolayers on permeable membranes, maintaining the cell polarity. Moreover the cells in culture produce cerebrospinal fluid.

Keywords

Phase Contrast Microscopy Choroid Plexus Confluent Monolayer Apical Chamber Epithelial Cell Culture 
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Résumé

Nous avons mis en place un modèle cellulaire in vitro de la barrière entre le sang et le liquide cérébrospinal à partir de cultures de cellules épithéliales du plexus choroïde de porc.Ces cultures conservent leurs propriétés spécifiques de cellules épithéliales et forment des monocouches cellulaires confluentes sur des supports perméables. De plus, ces cellules en culture sécrètent du liquide céphalo-rachidien.

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References

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Ursula Gath
    • 1
  • Ansgar Hakvoort
    • 1
  • H.-J. Galla
    • 1
  1. 1.Institut für BiochemieMünsterGermany

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