Summary
Measurement of transepithelial electrical resistances (TER) is a very common tool for investigations concerning epithelial and endothelial barrier properties. We here introduce two new and powerful techniques for the determination of TERs that might open further applications. The first method takes advantage of the wide spread DC technique and allows to study individual colonies of a cell monolayer grown on waterpermeable filter supports. The second technique is based on AC impedance analysis and enables to determine TERs of cell monolayers grown on gold surfaces.
Résumé
La mesure de la résistance transépithéliale (RTE) est un outil très courant pour la détermination des propriétés des barrières physiologiques épithéliales et endothéliales. Nous avons mis au point deux nouvelles techniques efficaces de mesure des RTE qui, de plus, pourraient permettre de nouvelles applications. La première méthode consiste à utiliser la technique bien connue en courant continu et permet la mesure de la RTE d’un petit nombre de cellules organisées en colonies distinctes sur des supports semi-imperméables. La seconde technique utilise l’analyse de l’impédance en courant alternatif et permet de déterminer les RTE de monocouches cellulaires ensemencées sur des supports recouverts d’or.
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References
L Hein M., Mädefesscl C., Haag B., Teichmann K., Post A., Galla H.-J., Chem. Phys. Lipids, 63 (1992) 223–233
Risau W., Dingier A., Albrecht U., Dehouck M.P., Ceccelli R.J., Neuochem., 58 (1992) 667–672
Erben M., Decker S., Franke H., Galla H.-J., J. Biochem. Biophys. Methods, in press
Stevenson B.R., Anderson J.M., Goodenough D.A., Mooseker M.S., Cell Biol., 107 (1988) 2401–2408
Richardson J.C.W., Scalera V., Simmons N.L., Biochim. Biophys. Acta, 673 (1981) 26–36
Griepp E.B., Dolan W.J., Robbins E.S., Sabatini D.D., J. Cell Biol., 96 (1983) 693–702
Erben M., Dissertation, Universität Münster, Germany, 1993
Rubin L.L. et al., J. Cell Biol., 155: 6 (1991) 1725–1735
Rutten M. J., Hoover R. J., Karnovsky M. J., Brain Res., 425 (1987) 301–310
Southwell B. et al., Endocrinology, 132 (1993) 2116–2126
Hein M., Post A., Galla H.-J., Chem. Phys. Lipids 63 (1992) 213–222
Kachar, Reese, Nature 296(1982)464–466
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© 1996 Springer Science+Business Media New York
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Wegener, J., Franke, H., Decker, S., Erben, M., Galla, HJ. (1996). New Techniques to Study Transepithelial and Transendothelial Resistances of Cultured Cells. In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_24
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DOI: https://doi.org/10.1007/978-1-4757-9489-2_24
Publisher Name: Springer, Boston, MA
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