Blood-Brain Barrier in Vitro

Rapid Evaluation of Strategies for Achieving Drug Targeting to the Central Nervous System
  • M. P. Dehouck
  • B. Dehouck
  • L. Fenart
  • R. Cecchelli
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)

Summary

To provide an in vitro system for studying brain capillary functions, we have developed a process of coculture that closely mimics the in vivo situation by culturing brain capillary endothelial cells on one side of a filter and astrocytes on the other side. The strong correlation between the in vivo (Oldendorfmethod) and in vitro (coculture) drug transport, the relative ease with which such cocultures can be produced in large quantities and the reproducibility of the system provide an efficient system for the screening of centrally active drugs.

Keywords

Drug Transport Nous Avons Brain Endothelial Cell Coculture Model Pinocytotic Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

De façon à étudier les fonctions des capillaires cérébraux support de la barrière hémato-encéphalique (BHE), nous avons mis au point un modèle de BHE in vitro qui consiste en une coculture de cellules endothéliales de capillaires cérébraux et d’astrocytes de part et d’autre d’un filtre. La parfaite corrélation entre les résultats obtenus pour le transport des médicaments in vivo (méthode d’Oldendorf) et in vitro (coculture), la facilité avec laquelle les cocultures peuvent être produites en grandes quantités et la reproductibilité du modèle permettent l’utilisation de ce modèle pour le screening des médicaments à visée cérébrale.

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References

  1. 1.
    Dehouck M.P., S. Méresse, P. Delorme, J.C. Fruchart and R. Cecchelli An easier, reproducible, and mass-production method to study the BBB in vitro. J. Neurochem., 54 1798–1801 (1990)PubMedCrossRefGoogle Scholar
  2. 2.
    Dehouck M.P., P. Jolliet-Riant, F. Brée, J.C. Fruchart, R. Cecchelli and J.P. Tillement Drug transfer across the BBB:correlation between in vitro and in vivo models. J. Neurochem., 58 1790–1797 (1992)PubMedCrossRefGoogle Scholar
  3. 3.
    Dehouck M.P., B. Dehouck, C. Schluep, M. Lemaire and R. Cecchelli Drug transport to the brain:comparison between in vitro and in vivo models of the BBB. European J. of Pharmaceutical Research, in press (1995)Google Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • M. P. Dehouck
    • 1
  • B. Dehouck
    • 1
  • L. Fenart
    • 1
  • R. Cecchelli
    • 1
    • 2
  1. 1.Institut PasteurINSERM U325LilleFrance
  2. 2.Villeneuve d’AscqUniversite De Lille IFrance

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