Summary
To provide an in vitro system for studying brain capillary functions, we have developed a process of coculture that closely mimics the in vivo situation by culturing brain capillary endothelial cells on one side of a filter and astrocytes on the other side. The strong correlation between the in vivo (Oldendorfmethod) and in vitro (coculture) drug transport, the relative ease with which such cocultures can be produced in large quantities and the reproducibility of the system provide an efficient system for the screening of centrally active drugs.
Résumé
De façon à étudier les fonctions des capillaires cérébraux support de la barrière hémato-encéphalique (BHE), nous avons mis au point un modèle de BHE in vitro qui consiste en une coculture de cellules endothéliales de capillaires cérébraux et d’astrocytes de part et d’autre d’un filtre. La parfaite corrélation entre les résultats obtenus pour le transport des médicaments in vivo (méthode d’Oldendorf) et in vitro (coculture), la facilité avec laquelle les cocultures peuvent être produites en grandes quantités et la reproductibilité du modèle permettent l’utilisation de ce modèle pour le screening des médicaments à visée cérébrale.
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References
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© 1996 Springer Science+Business Media New York
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Dehouck, M.P., Dehouck, B., Fenart, L., Cecchelli, R. (1996). Blood-Brain Barrier in Vitro . In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_23
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DOI: https://doi.org/10.1007/978-1-4757-9489-2_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9491-5
Online ISBN: 978-1-4757-9489-2
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