Membrane Fractionation of Brain Capillary Endothelial Cells and Analysis of Lipid Polarity

  • Bernhard J. Tewes
  • Hans-Joachim Galla
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)


Membrane fractionation of cultured porcine brain capillary endothelial cells (BCEC) was performed and the phospholipid composition of the obtained membrane fractions was analyzed. Strong differences between the apical and the basolateral membrane domains were observed for phosphatidylcholine pointing out that membrane lipids are polar distributed in BCEC.


Lipid Polarity Apical Membrane Basolateral Membrane Phospholipid Composition High Performance Thin Layer Chromatography 
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Nous avons étudié la composition en phospholipides des membranes de cultures de cellules endothéliales de microvaisseaux cérébraux de porc, après fractionnement membranaire.Nous avons observé que la composition des membranes apicales et basolatérales en phosphatidylcholine était différente, suggérant que la distribution membranaire des lipides est polarisée dans les cellules endothéliales de capillaires cérébraux de porc établies en culture.


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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Bernhard J. Tewes
    • 1
  • Hans-Joachim Galla
    • 1
  1. 1.Department of BiochemistryUniversity of MuensterMuensterGermany

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