Aryl Hydrocarbon Receptor mRNA Levels in Different Tissues of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin-Responsive and Nonresponsive Mice
The AhR mRNA contents were measured in different tissues of 2,3,7,8-tetrachlo-rodibenzo-p-dioxin (TCDD)-responsive C57BL/6J and nonresponsive DBA/2J mice. Out of all examined tissues the highest AhR mRNA levels were found in lung, with concentrations of 31.7 ± 11.0 × 103 molecules / 100 ng RNA and 20.3 ± 8.9 × 103 molecules / 100 ng RNA in C57BL/6J and DBA/2J mice, respectively. The AhR mRNA contents were 5–10 fold lower in heart, liver, thymus, brain and placenta. Low levels were found in spleen, kidney and muscle. Since no significant differences in AhR mRNA expression between the two strains were observed, factors other than regulation of AhR gene expression seem to be responsible for the observed different susceptibility toward TCDD.
KeywordsAryl Hydrocarbon Receptor Concentration Of31 Aryl Hydrocarbon Receptor Agonist Competitive Polymerase Chain Reaction Aryl Hydrocarbon Receptor Binding
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