Development of a Mass Spectrometric Assay for 5,6,7,9-Tetrahydro-7-Hydroxy-9-Oximidazo [1,2-a]Purine in DNA Modified by 2-Chloro-Oxirane
Vinyl chloride is recognized as a human carcinogen. 2-Chlorooxirane, its biological reactive intermediate formed by the P450 enzyme system in the liver, and the rearrangement product chloroacetaldehyde give rise to the etheno (ε) DNA adducts. This type of modified DNA bases appears to be the molecular basis for the genotoxic effects of the parent compound, and site specific mutagenesis studies (Basu et al., 1993) show its mutagenicity. There is evidence that ε adducts might not only be formed by vinyl chloride metabolites but also be present at a certain level due to endogeneous sources (Misra et al., 1994).
KeywordsVinyl Chloride Selective Reaction Monitoring Heavy Isotope Mass Spectrometric Assay Negative Electrospray Ionization Mass
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