Abstract
The basic principle of energy transduction in living systems is to create a transmembrane electric potential difference and pH gradient, the so called electrochemical energy, by proton translocation [1], Bacteriorhodopsin (BR), the protein in the plasma membrane of Halobacterium halobium turned out to be a very important molecule in understanding the mechanism of proton translocation [2]. Its main features are:
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it is a protein-pigment complex with retinal chromophore, similar to eye-rhodopsin,
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undergoes a cyclic reaction pathway after absorbing a photon, and
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this photocycle is connected with proton pumping from the inside of the cell to the outside.
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References
P. Boyer, B. Chance, L. Ernster, P. Mitchell, E. Racker and E. Slater, Annu. Rev. Biochem., 46, 966 (1977)
W. Stoeckenius, R.H. Lozier and R.A. Bogomolni, Biochim. Biophys. Acta, 505, 225 (1979).
D. Oesterhelt and W. Stoeckenius, Proc. Natl. Acad. Sci. USA, 70, 2853 (1973).
Yu. A. Ovchinnikov, N.G. Abdulaev, M.Y. Feigina, A.V. Kiselev and N.A. Lobanov, FEBS Lett., 100, 219 (1979).
H.G. Khorana, G.E. Gerber, W.C. Herlihy, C.P. Gray, R.J. Anderegg, K. Nihei and K. Biemann, Proc. Natl. Acad. Sci. USA, 76, 5046 (1979).
S. Das Sarma, K.L. Raj Bhandary and H.G. Khorana, Proc. Natl. Acad. Sci. USA, 81, 125 (1984).
T. Mogi, L.J. Stern, T. Marti, B.H. Chao and H.G. Khorana, Proc. Natl. Acad. Sci. USA, 85, 4148 (1988).
R. Henderson and P.N.T. Unwin, Nature London, 251, 28 (1975).
R. Henderson, J.M. Baldwin, K. Downing, J. Lepault and F. Zemlin, Eur. J. Cell, 44, 23 (1987).
F. Seiff, J. Wallant, P. Ermann and M.P. Heyn, Proc. Natl. Acad. Sci. USA, 82, 3227 (1985).
F. Seiff, J. Westerhausen, J. Wallat and M.P. Heyn, Proc. Natl. Acad. Sci. USA, 83, 7746 (1986).
D. Oesterhelt and W. Stoeckenius, Meth. Enzymol., 31, 667 (1974).
J.K. Lányi and V. Vodyanoy, Biochemistry, 25, 1465 (1986).
J.L. Spudich and R.A. Bogomolni, in Biophysical Studies of Retinal Proteins, T.G. Ebrey, H. Frauenfelder, B. Honig and K. Nakanishi (Editors) University of Illinois Press, (1987), p. 24.
E. Racker and W. Stoeckenius, J. Biol. Chem., 249, 1955 (1974).
N.A. Dencher and M.P. Heyn, FEBS Lett., 108, 307 (1979).
R. Henderson, J.M. Baldwin, T.A. Aske, R.M. Glaser and I.N. Tsygannik, in Retinal Proteins, Yu. A. Ovchinnikov (Editor), VNU Science Press, Utrecht, (1987), p. 153.
P. Scherrer, W. Stoeckenius, M.K. Mathew and W. Sperling in Biophysical Studies of Retinal Proteins, T.G. Ebrey, H. Frauenfelder, B. Honig and K. Nakanishi (Editors), University of Illinois Press, (1987), p. 206.
S.O. Smith, A.B. Myers, J.A. Pardoen, C. Winkler, P.P.J. Mulder, J. Lugtenburg and R. Mathies, Proc. Natl. Acad. Sci. USA, 81, 2055 (1984).
S.O. Smith, M.S. Braiman, A.B. Myers, J.A. Pardoen, J.M.L. Courtin, C. Winkel, J. Lugtenburg and R.A. Mathies, J. Am. Chem. Soc., 109, 3108 (1987).
G.S. Harbison, S.O. Smith, J.A. Pardoen, J.M.L. Courtin, J. Lugtenburg, J. Hertzfeld, R.A. Mathies and R.G. Griffin, Biochemistry, 24, 695 (1985).
J.L. Spudich, D.A. McCain, K. Nakanishi, M. Okabe, N. Shimizu, H. Rodman, B. Honig and R.A. Bogomolni, Biophys. J., 49, 479 (1986).
P. Hildebrandt and M. Stockburger, Biochemistry, 23, 5539 (1984).
R.H. Lozier, R.A. Bogomolni and W. Stoeckenius, Biophys. J., 15, 955 (1975).
S.O. Smith, J. Lugtenburg and R.A. Mathies, J. Membr. biol., 85, 95 (1985).
K. Bagley, G. Dollinger, L. Eisenstein, A.K. Singh and L. Zimányi, Proc. Natl. Acad. Sci. USA, 79, 4972 (1982).
B. Hess and D. Kuschmitz, FEBS Lett., 100, 334 (1979).
G. Dollinger, L. Eisenstein, S.L. Lin, K. Nakanishi and J. Termini, Biochemistry, 25, 6524 (1986).
M. Engelhard and B. Hess, in Retinal Proteins, Yu. A. Ovchinnikov, (Editor), VNU Science Press, Utrecht, (1987), p. 259.
H.W. Trissl and M. Montal, Nature (London), 266, 655 (1977).
L.A. Drachev, A.D. Kaulen and V.P. Skulachev, FEBS Lett., 87, 161 (1978).
A. Fahr, P. Läuger and E. Bamberg, J. Membr. Biol., 60, 51 (1981).
G.W. Rayfield, Biophys. J., 48, 111 (1985).
L. Keszthelyi and P. Ormos, FEBS Lett., 109, 189 (1980).
Zs. Dancsházy, R. Govindjee and T.G. Ebrey, Proc. Natl. Acad. Sci. USA, 85, 6358 (1988).
L.A. Drachev, A.D. Kaulen, V.P. Skulachev and V.V. Zorina, FEBS Lett., 226, 139 (1987).
T. Kouyama, A. Nasuda-Kouyama, A. Ikegemi, M.K. Mathew and W. Stoeckenius, Biochemistry, 27, 5855 (1988).
H.J. Polland, M.A. Franz, W. Zinth, W. Kaiser, E. Kölling and D. Oesterhelt, Biophys. J., 49, 651 (1986).
B. Hess and D. Kuschmitz, FEBS Lett., 100, 334 (1979).
G. Dollinger, L. Eisenstein, S.L. Lin, K. Nakanishi and J. Termini, Biochemistry, 25, 6524 (1986).
M. Engelhard and B. Hess, in Retinal Proteins, Yu. A. Ovchinnikov, (Editor), VNU Science Press, Utrecht, (1987), p. 259.
H.W. Trissl and M. Montal, Nature (London), 266, 655 (1977).
L.A. Drachev, A.D. Kaulen and V.P. Skulachev, FEBS Lett., 87, 161 (1978).
A. Fahr, P. Läuger and E. Bamberg, J. Membr. Biol., 60, 51 (1981).
G.W. Rayfield, Biophys. J., 48, 111 (1985).
L. Keszthelyi and P. Ormos, FEBS Lett., 109, 189 (1980).
Zs. Dancsházy, R. Govindjee and T.G. Ebrey, Proc. Natl. Acad. Sci. USA, 85, 6358 (1988).
L.A. Drachev, A.D. Kaulen, V.P. Skulachev and V.V. Zorina, FEBS Lett., 226, 139 (1987).
T. Kouyama, A. Nasuda-Kouyama, A. Ikegemi, M.K. Mathew and W. Stoeckenius, Biochemistry, 27, 5855 (1988).
H.J. Polland, M.A. Franz, W. Zinth, W. Kaiser, E. Kölling and D. Oesterhelt, Biophys. J., 49, 651 (1986).
E. Bamberg and K. Fendler, in this volume, Chapter 4.
L. Keszthelyi and P. Ormos, Biophys. Chem., 18, 397 (1983).
E. Frederiq and C. Houssier: Electric Dichroism and Electric Birefringence. Clarendon Press, Oxford, (1973), p. 81.
M.I. Shah, J. Phys. Chem.67, 2215 (1963).
P. Ormos, L. Reinisch and L. Keszthelyi, Biochim. Biophys. Acta, 722, 471 (1983).
A. Dér, P. Hargittai and J. Simon, J. Biochem. Biophys. Meth., 10, 295 (1985).
G. Váró, Acta Biol. Acad. Sci. Hung., 32 301 (1982).
L. Keszthelyi, in Information and Energy Transduction in Biological Membranes, L. Bolis. E.J. Helmreich and H. Passow, (Editors), Alan R. Liss, Inc. New York, (1984), p. 51.
P. Ormos, S.G. Hristova and L. Keszthelyi, Biochim. Biophys. Acta, 809, 181 (1985).
K.H. Müller, H.J. Butt, M. Engelhard, B. Hess and E. Bamberg, in Molecular Physiology of Retinal Proteins, T. Hara, (Editor), Yamada Sci. Found., (1988), p. 341.
P. Läuger, R. Benz, G. Stark, E. Bamberg, P.C. Jordan, A. Fahr and V. Brock, Q. Rev. Biophys., 14, 513 (1981).
L. Keszthelyi and P. Ormos, Biophys. Chem., 18, 397 (1983).
G. Groma, F. Ráksi, G. Szabó and G. Váró, Biophys. J., 54, 77 (1988).
H.D. Zwolinsky, H. Eyring and C.E. Reece, J. Phys. Chem., 53, 1426 (1949).
D. Braun, N.A. Dencher, A. Fahr, M. Lindau and M.P. Heyn, Biophys. J., 53, 617 (1988).
P. Scherrer, L. Packer and S. Seltzer, Arch. Biochem. Biophys., 212, 589 (1981).
L. Packer, P. Scherrer, K.T. Yue, Gy. Váró, P. Ormos, K. Barabás, A. Dér and L. Keszthelyi, Biochem. Int., 5, 437 (1982).
S. Wu-Chou, A.E. Robinson, E. Hrabeta and L. Packer, Biochem. Biophys. Res. Comm., 124, 556 (1984).
L. Packer, E. Hrabeta-Robinson, S.G. Taneva, R. Tóth-Boconádi and L. Keszthelyi, Biochem. Int., 14, 977 (1987).
R. Tóth-Boconádi, S.G. Taneva, A.V. Kiselev, N.G. Abdulaev and L. Keszthelyi, Arch Biochem. Biophys., 260, 726 (1988).
A.V. Kiselev, N.G. Abdulaev, R.G. Vasilov, L.R. Nabiev and Yu.A. Ovchinnikov, in Short Reports of Third European Bioenergetics Conference, Congress Edition Hannover, 3A, 27 (1984).
N.G. Abdulaev, N.A. Dencher, A.E. Dergachev, A. Fahr and A.V. Kiselev, Biophys. Struct Mech, 10, 211 (1984).
G. Váró and L. Keszthelyi, Biophys. J., 43, 47 (1983).
G. Váró and L. Keszthelyi, Biophys. J., 47, 243 (1985).
G. Váró and L. Eisenstein, Eur. Biophys J., 14, 163 (1987).
R. Tóth-Boconádi, S.G. Hristova and L. Keszthelyi, FEBS Lett., 19, 164 (1986).
A. Dér, R. Tóth-Boconádi and L. Keszthelyi, FEBS Lett., 228, 313 (1988).
J.F. Nagle and S. Tristram-Nagle, J. Memhr. Biol., 74, 1 (1983).
A. Brunger, Z. Schulten and K. Schulten, . Z. Phys. Chem., 135, 1 (1983).
H. Merz and G. Zundel, Biochem. Biophys. Res. Comm., 101, 540 (1981).
B.J. Plotkin and W.V. Sherman, Biochemistry, 23, 5353 (1984).
A. Dér, K. Fendler, S. Száraz, L. Keszthelyi, D. Oesterhelt and E. Bamberg, Biochim. Biophys. Acta, in the press (1988).
P.L. Ahl, L.J. Stern, N.R. Hackett, K.J. Rotschild and H.G. Khorana, Biophys. J., 51 416a (1987).
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Keszthelyi, L. (1990). Bacteriorhodopsin-Structure and Function. In: Milazzo, G., Blank, M. (eds) Bioelectrochemistry III. Ettore Majorana International Science Series, vol 51. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9459-5_8
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