Ovule Culture to Study Reproductive Development at the Cellular Level in Loblolly Pine (Pinus taeda L.)

  • Deneke H. Mariam
  • David L. Bramlett
  • John E. Mayfield
  • William V. Dashek
Part of the Biodeterioration Research book series (BIOR, volume 3)


Loblolly pine is an important commercial timber and pulpwood tree of the Southeastern United States (Guiness, 1982). Recently, inadequate regeneration of this pine was identified as a problem causing reduction in the volume of softwood in the Southeast. To supply adequate seedlings for regeneration, nurseries (Davey, 1982) and clonal seed orchards (Zobel et al., 1958) have been established. Successful plantations of loblolly pine can be established from nursery-grown seedlings of improved genetic strains (Dierauf, 1982). To fully understand seed development in loblolly pine (Figure 1), the culture of excised ovules could be used to study in vitro fertilization. If excised loblolly pine ovules can be maintained in culture, the production of plantlets could perhaps be enhanced by methods similar to existing procedures (Mott and Anderson, 1981).


Cell Length Seed Orchard Cell Width Ovule Culture Clonal Seed Orchard 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Deneke H. Mariam
    • 1
  • David L. Bramlett
    • 1
  • John E. Mayfield
    • 2
  • William V. Dashek
    • 1
  2. 2.Biology DepartmentNorth Carolina Central UniversityDurhamUSA

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