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Immunological Characterization of Fungal Enzymes and Biological Chelators Involved in Lignocellulose Degradation

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Biodeterioration Research

Part of the book series: Biodeterioration Research ((BIOR,volume 3))

Abstract

Understanding the basic mechanisms underlying wood decay is currently of interest because of the need for decay prevention in in-service wooden structures. Additional interest stems from the potential use of biological processes to modify or alter wood, and thereby produce useful chemicals, fuels, pulp, or other materials. Although cellulose and lignin (the basic components of wood) are the two most abundant organic materials on earth, still little is known about the mechanisms involved in their degradation. It has long been postulated that enzymes are involved in the decay process, but the first ligninase from a white-rot fungus was isolated for characterization only as recently as 1983 (Tien and Kirk). More attention has been focused on this field in the last six years but still little is known about enzymes and other metabolites from wood degrading fungi. This is particularly true with regard to how, and if, both cellulose and lignin destroying enzymes penetrate into the wood substance to cause degradation.

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References

  • Blanchette, R.A., Abad, A.R., Cease, K.R., Lovrien, R.E. and Leathers, T.D. (1989a). Colloidal gold cytochemistry of endo 1, 4-β-glucanase, l, 4-β-D glucan cellobiohydrolase and endo 1, 4-β-xylanase: Ultrastructure of sound and decayed birch wood. Appl. Environ. Microbiol., in press.

    Google Scholar 

  • Blanchette, R.A., Abad, A.R., Farrell, R.L., and Leathers, T.D. (1989b). Detection of lignin peroxidase and xylanase by immunocytochemical labelling in wood decayed by basidiomycetes. Appl. Environ. Microbiol., in press.

    Google Scholar 

  • Broda, P., Raeder, U., Brown, A., Thompson, W., Schrank, A., and Simms, P. (1987). Molecular genetics of lignin biodegradation. In: FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France. Abst. L-23.

    Google Scholar 

  • Carpita, N., Sabularse, D., Montezinos, D., and Delmer, D.P. (1979). Determination of the pore size of cell walls of living plant cells. Science, 205, 1144–1147.

    Article  CAS  Google Scholar 

  • Daniel, G.F., Nilsson, T., and Pettersson, B. (1988). Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium. The International Working Group on Wood Preservation. Working Group la. Prepared for the 19th Annual Meeting, Madrid, Spain. Document IRG/WP 1364.

    Google Scholar 

  • Dolphin, D. (1986). Porphyrin enzymes and their relation to lignin biodegradation. Talk presented Nov. 20, 1986 at the Biofor II Workshop on Ligno-Cellulose Enzymology, Victoria, B.C.

    Google Scholar 

  • Dolphin, D., Kirk, T.K., and Farrell, R. (1987). Synthetic Model Ligninases. International Seminar on Lignin Enzymic and Microbial Degradation. April 23–24, 1987. Paris, France. Abst. p. 12.

    Google Scholar 

  • Enari, T.-M. (1985). Biotechnology, a challenge and an opportunity for the forest products industry. The Marcus Wallenberg Foundation Symposia Proceedings, 2, 54-67. Falun, Sweden.

    Google Scholar 

  • Farrell, R.L., Maione, T.E., and Sadownick, B.A. (1987). Activities of Recombinant Ligninase. FEMS Symposium, Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France, Abst. L-24.

    Google Scholar 

  • Fekete, F., Chandhoke, V., and Jellison, J. (1989). Iron-binding compounds produced by wood decaying basidiomycetes. Appl. Environ. Microbiol., accepted.

    Google Scholar 

  • Fekete, F.A., Spence, J.T., and Emery, T. (1983). A rapid and sensitive paper electrophoresis assay for the detection of microbial siderophores elicited in solid-plating culture. Anal. Biochem., 131, 516–519.

    Article  CAS  Google Scholar 

  • Forney, L.J., Reddy, C.A., and Pankratz, H.S. (1982). Ultrastructural localization of hydrogen peroxide production in ligninolytic Phanerochaete chrysosporium cells. Appl. Environ. Microbiol., 44, 732–736.

    CAS  Google Scholar 

  • Garcia, S., Latge, J.P., Prevost, M.C., and Leisola, M. (1987a). Cytochemical study of wood degradation by white rot fungi using lignin peroxidase immunoglobulin-gold complexes. Appl. Environ. Microbiol., 53, 2384–2387.

    CAS  Google Scholar 

  • Garcia, S., Latge, J.P., Prevost, M.C., and Leisola, M. (1987b). Cytochemical study of wood degradation by Phanerochaete chrysosporium. In: International Seminar on Lignin Enzymic and Microbial Degradation. April 23–24, 1987. Paris, France. Abst. p. 38.

    Google Scholar 

  • Glenn, J.F., and Gold, M.H. (1985). Purification and characterization of an extracellular Mn(II)-dependent peroxidase from the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Arch. Biochem. Biophysics, 242, (2) 329–341.

    Article  CAS  Google Scholar 

  • Goodell, B.S., and Jellison, J. (1986). Detection of a brown-rot fungus using serological assays. The International Research Group on Wood Perservation. Document IRG/WP 1305.

    Google Scholar 

  • Goodell, B., Jellison, J., and Daniel, G. (1989). Probing peroxidase activity in Phanaerochaete chrysosporium degraded birch wood. Presented at the Fourth International Symposium on Biotechnology in the Pulp and Paper Industry, Raleigh, N.C., May 16–19. Abstr. 2 pp.

    Google Scholar 

  • Hider, R.C. (1984). Siderophore mediated absorption of iron. Struct. Bonding, 58, 25–87.

    Article  CAS  Google Scholar 

  • Highley, T.L. (1980). Degradation of cellulose by Poria placenta in the presence of compounds that affect hydrogen peroxide. Material und Organismen, 15, 81–90.

    CAS  Google Scholar 

  • Illman, B.L., Meinholtz, D.C, and Highley, T.L. (1988a). An electron spin resonance study of managanese changes in wood decayed by the brown-rot fungus, Postia placenta. The International Research Group on Wood Preservation, Madrid, Spain. Document IRG/WP 1359.

    Google Scholar 

  • Illman, B.L., Meinholtz, D.C., and Highley, T.L. (1988b). Generation of hydroxyl radicals by the brown-rot fungus, Postia placenta. The International Research Group on Wood Preservation, Madrid, Spain. Document IRG/WP 1360.

    Google Scholar 

  • Jellison, J., and Goodell, B.S. (1986). Production of monoclonal antibodies to fungal metabolites. The International Research Group on Wood Preservation No. IRG/WP.

    Google Scholar 

  • Jellison, J., and Goodell, B. (1988). Immunochemical characterization of lignocellulose degradation. J. Biomass, 15, 109–116.

    Article  CAS  Google Scholar 

  • Kirk, T.K. (1988). Personal communication. University of Maine, Orono, ME, US. 10/1988.

    Google Scholar 

  • Kirk, T.K., and Cowling, E.B. (1984). Biological decomposition of solid wood. In: The Chemistry of Solid Wood. pp. 455-488 (R. Rowell, ed.), American Chemical Society Advances in Chemistry. Series 207. Washington, D.C.

    Google Scholar 

  • Kirk, T.K., Croan, S., and Tien, M. (1986). Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strain. Enzyme Microbial. Technol., 8, 27–32.

    Article  CAS  Google Scholar 

  • Kirk, T.K., and Tien, M. (1986). Lignin degrading activity of Phanerochaete chrysosporium Burds.: comparison of cellulase-negative and other strains. Enzyme Microbial. Technol., 8, 75–80.

    Article  CAS  Google Scholar 

  • Knowles, J.K.C., Lehtovaara, P., Teeri, T.T., Penttila, M., and Niku-Paavola, M.L. (1987). The use of gene technology to investigate the mode of action of fungal cellulolytic enzymes. In: FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France. Abst. L-10.

    Google Scholar 

  • Koenigs, J.W. (1974). Hydrogen peroxide and iron: A proposed system for decomposition of wood by brown-rot basidiomycetes. Wood Fiber, 6, 66–80.

    Google Scholar 

  • Leisola, M., Haemmerli, S., Smit, J.D.G., Troller, J., Waldner, R., Schoemaker, S., and Schmidt, H. (1987). Phanerochaete chrysosporium: characterization of ligninolytic enzymes and metabolism of veratryl alcohol. International Seminar on Lignin Enzymic and Microbial Degradation, April 23–24, 1987. Paris, France. Abst. p. 6.

    Google Scholar 

  • McHale, A.P. (1987). Production and characterization of monoclonal antibodies to the cellulases produced by Talaromyces emersonni CBS 814.70. Biochem. Biophys. Acta, 924, 147–153.

    Article  CAS  Google Scholar 

  • Messner, R., and Kubicek, C.P. (1987). Intracellular precursers of Endo-β-1, 4-glucanase in Trichoderma reesei. In: FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France. Abst. P1-27.

    Google Scholar 

  • Messner, K., Srebotnik, E., Ertler, G., Foisner, R., and Stachelberger, H. (1987). Cell wall systems and extracellular membraneous structures of white rot fungi. In: International Seminar of Lignin Enzymic and Microbial Degradation. April 23–24, 1987. Paris, France. Abst. p. 31.

    Google Scholar 

  • Micales, J.A., and Highley, T.L. (1988). The effect of tunicamycin on production and secretion of extracellular carbohydrate-degrading enzymes by Postia placenta. Doc. No. IRG/WP/1342. The International Research Group on Wood Preservation, Working Group la, April 24–29, Madrid, Spain.

    Google Scholar 

  • Morgenstern, E., Bayer, E.A., Vliegenthart, J.F.G., Gerwig, G., Kamerling, H., and Lamed, R. (1987). Structure and possible function of glycosylated polypeptides in Clostridium thermocellum. In: FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France. Abst. P2-06.

    Google Scholar 

  • Murmanis, L., Highley, T.L., and Palmer, J.G. (1988). The action of isolated brown-rot cell-free culture filtrate, H2O2-Fe++, and the combination of both on wood. Wood Sci. Technol., 22, 59–66.

    Article  CAS  Google Scholar 

  • Neilands, J.B. (1974). Iron and its role in microbial physiology, In: Microbial Iron Metabolism: A Comprehensive Treatise, pp. 3–34 (J.B. Nielands, ed.), Academic Press, Inc., New York.

    Google Scholar 

  • Neilands, J.B., Konopka, K., Schwyn, B., Coy, M., Francis, R.T., Paw, B.H., and Bagg, A. (1987). Comparative biochemistry of microbial iron assimilation, In: Iron Transport in Microbes, Plants and Animals. p. 3–33 (G. Winkelmann, D. van der Helm, and J.B. Neilands, eds.), VCH Publishers, New York.

    Google Scholar 

  • Paszcynski, A., Crawford, R.L., and Blanchette, R.A. (1988). Delignification of wood chips and pulps by using natural and synthetic porphyrins: models of fungal decay. Appl. Environ. Microbiol., 54, 62–68.

    Google Scholar 

  • Paszczynski, A., Huynh, V.-B., and Crawford, R. (1986). Comparison of ligninase-1 and peroxidase-M2 from the white-rot fungus Phanerochaete chrysosporium. Archives of Biochem. and Biophysics, 244(2), 750–765.

    Article  CAS  Google Scholar 

  • Popp, J.L., and Kirk, T.K. (1989). Unpublished. Fourth International Conference, Biotechnology in the Pulp and Paper Industry, May 16–19, Raleigh, NC.

    Google Scholar 

  • Renganathan, V., Miki, K., and Gold, M.H. (1985). Multiple molecular forms of diarylpropane oxygenase, an H2O2-requiring, lignin-degrading enzyme from Phanerochaete chrysosporium. Arch. Biochem. Biophys., 241(1), 304–314.

    Article  CAS  Google Scholar 

  • Schmuck, M., Pilz, I., Hayn, M., and Esterbauer, H. (1986). Investigation of cellobiohydralase from Trichoderma reesei by small angle X-ray scattering. Biotechnol. Letters, 86(6), 397–402.

    Article  Google Scholar 

  • Schwyn, B., and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal. Biochem., 160, 46–56.

    Article  Google Scholar 

  • Shoemaker, S.P. (1985). Future protein engineering approaches as applied to wood and wood products. The Marcus Wallenberg Foundation Symposia Proceedings, 2, 43–53.

    Google Scholar 

  • Sims, P, and Broda, P. (1987). The identification, molecular cloning and characterization of a gene from Phanerochaete chrysosporium that shows strong homology to the exo-cellobiohydrolase I gene from Trichoderma reesei. In: FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. Sept. 7–9, 1987. Institut Pasteur, Paris, France. P3-24.

    Google Scholar 

  • Srebotnik, E., and Messner, K. (1988). Spatial arrangement of lignin peroxidase in pine decayed by Phanerochaete chrysosporium and Fomitopsis pinicola. The International Research Group on Wood Preservation. Working Group Ia. Biological Problems (Flora). Document IRG/WP.

    Google Scholar 

  • Srebotnik, E., Messner, K., and Foisner, R. 1988. Penetrability of white rot-degraded pine wood by the lignin peroxidase of Phanerochaete chrysosporium. Appl. Environ. Microbiol., 54, 11, 2608, and 2883.

    Google Scholar 

  • Tepfer, M. and I. E. P. Taylor (1979). The permeability of plant cell walls as measured by gel filtration chromatography. Science, 213, 761–763.

    Article  Google Scholar 

  • Tien, M., and Kirk, T.K. (1983). Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science, 221, 661–662.

    Article  CAS  Google Scholar 

  • Tien, M., and Tu, C.P.D. (1987). Cloning and sequencing of cDNA for a ligninase from Phanerochaete chrysosporium. Nature, 326, 612.

    Article  Google Scholar 

  • Warren, A. (1986). Cloning of cellulose genes from Cellulommas fimi. Talk presented Nov. 20, 1986 at the Biofor II Workshop on LignoCellulose Enzymology, Victoria, B.C.

    Google Scholar 

  • Westervelt, P., Bloom, M.L., Mabbott, G.A., Fekete, F.A. (1985). The isolation and identification of 3, 4-dihydroxybenzoic acid formed by nitrogen-fixing Azomonas macrocytogens. FEMS Microbiology letters, 30, 331–335.

    Article  CAS  Google Scholar 

  • Wolter, K.E., Highley, T.L., and Evans, F.J. (1980). A unique polysaccharide and glycoside-degrading enzyme complex from the wood-decay fungus Poria placenta. Biomed. Biophys. Res. Comm., 97, 1499–1504.

    Article  CAS  Google Scholar 

  • Wood, M., McCrae, S.I., Wilson, CA., Bhat, K.M., Gow, L.A., and Manning, J.E. (1987). Aerobic and anaerobic fungal cellulases with special reference to their mode of attack on crystalline cellulose. FEMS Symp. Biochemistry and Genetics of Cellulose Degradation. September 7–9, 1987. Institut Pasteur, Paris, France. Abst. L-03.

    Google Scholar 

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Goodell, B., Jellison, J. (1990). Immunological Characterization of Fungal Enzymes and Biological Chelators Involved in Lignocellulose Degradation. In: Llewellyn, G.C., O’Rear, C.E. (eds) Biodeterioration Research. Biodeterioration Research, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9453-3_29

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  • DOI: https://doi.org/10.1007/978-1-4757-9453-3_29

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