Abstract
Our previous attempts to elucidate the structure and extent of the hyphal sheath of wood-decay fungi by scanning electron microscopy (SEM) showed that different preparative methodologies yield differing and frequently conflicting results. Foisner et al. (1985a and b), Highley and Murmanis (1985, 1987), and Green et al. (1989) provided substantial ultrastructural evidence for the presence of extracellular membranous structures that assume a variety of forms, including sheets, tubules, vesicles, and fibers. Evans et al. (1981) reported a fibrillar sheath surrounded by a tripartite pellicle on rapidly growing Bipolaris maydis. Day et al. (1986a and b) also provided evidence for extracellular fungal stuctures, called “fungal fimbriae”, which the authors described as primarily proteinaceous. Foisner et al. (1985a and b) analyzed isolated, extracellular membranous structures, which were reportedly composed of carbohydrate, protein, and lipid. These structures were better visualized by transmission electron microscopy (TEM) after treatment with osmium and/or ruthenium red.
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References
ASTM. (1971). Standard method for accelerated laboratory test of natural decay resistance of woods. American Society for Testing and Material Desig. D 2017, Philadelphia, PA.
Beckett, A., Porter, R., and Read, N.D. (1982). Low temperature scanning electron microscopy of fungal material. J. Microbiol., 125, 193–199.
Blanchette, R.A., Abad, A.R., Farrel, R.L., and Leathers, T.D. (1989). Detection of lignin peroxidase and xylanase by immunocytochemical labeling in wood decayed by Basidiomycetes. Appl. Environ. Microbiol., 55, 1457–1465.
Bonfante-Fasolo, P., Perotto, S., Testa, B., and Faccio, A. (1987). Ultrastructural localization of cell surface sugar residues in ericoid mycorrhizal fungi by gold-labeled lectins. Protoplasma, 139, 25–35.
Bracker, C.E. (1967). Ultrastructure of fungi. Ann. Rev. Phytopath., 5, 343–374.
Cole, G.T. (1986). Preparation of microfungi for scanning electron microscopy. In: Ultrastructure Techniques for Microorganisms, pp. 1–86, (Aldrich, H.C., and W.J. Todd, Eds.), Plenum Press, NY.
Crang, R.F.E. (1988). Artifacts in specimen preparation for scanning electron microscopy, In: Artifacts in Biological Electron Microscopy, pp. 107–129, (Crang, R.F.E., and K.L. Klomparens, Eds.), Plenum Press, NY.
Daniel, G., Nilsson, T., and Pettersson, B. (1989). Intra-and extracellular localization of lignin peroxidase during the degradation of solid wood and wood fragments by Phanerochaete chrysosporium by using transmission electron microscopy and immuno-gold labeling. Appl. Environ. Microbiol., 55, 871–881.
Day, A.W., Gardiner, B., Smith, R., Svircev, A.M., and McKeen, W.E. (1986a). Surface proteinaceous fibrils (fimbriae) on filamentous fungi. Can. J. Bot., 66, 2474–2484.
Day, A.W., Gardiner, B., Smith, R., Svircev, A.M., and McKeen, W.E. (1986b). Detection of fungal fimbriae by protein A-gold immunocytochemical labelling in host plants infected with Ustilago heufleri or Peronospora hyoscyami f. sp. tabacina. Can. J. Microbiol., 32, 577–584.
Evans, R.C., Stempen, H., and Stewart, S.J. (1981). Development of hyphal sheaths in Bipolaris maydis race T. Can. J. Bot., 59, 453–459.
Fleet, G.H., and Phaff, H.J. (1981). Fungal glucans-structure and metabolism, In: Plant Carbohydrates II. Extracellular Carbohydrates, pp. 416–440, (W. Tanner and F.A. Loewus, Eds.), Springer-Verlag, Berlin.
Foisner, R., Messner, K., and Roehr, M. (1985a). Wood decay by basidiomycetes: Extracellular tripartite membranous structures. Trans. Brit. Mycol. Soc., 85, 257–266.
Foisner, R., Messner, K., Stachelberger, H., and Roehr, M. (1985b). Isolation and characterization of extracellular three-lamellar structures of Sporotrichum pulverulentum. J. Ultrastruct. Res., 92, 36–46.
Green, F., Larsen, M.J., Murmanis, L.L., and Highley, T.L. (1989). Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia Placenta. Intern. Res. Group Wood Preserv., IRG Document. No. 1391. Stockholm.
Green, D.E. and Tzagoloff, A. (1966). Role of lipids in the structure and function of biological membranes. J. Lipid Res., 7, 587–602.
Handley, P.S. Hargreaves, J., and Harty, D.W.S. (1988). Ruthenium red staining reveals surface fibrils and a layer external to the cell wall in Streptococcus salivarius HB and adhesion deficient mutants. J. Gen. Microbiol., 134, 3165–3172.
Hayat, M.A. (1981). Fixation for Electron Microscopy, 501 pp., Academic Press NY.
Highley, T.L. (1987). Biochemical aspects of white-rot and brown-rot decay. Intern. Res. Group Wood Preserv., IRG Document. No. 1391.
Highley, T.L., and Murmanis, L.L. (1985). Micromorphology of degradation in western hemlock and sweetgum by the brown-rot fungus Poria placenta. Holzforschung, 39, 73–78.
Highley, T.L., and Murmanis, L.L. (1987). Micromorphology of degradation in western hemlock and sweetgum by the white-rot fungus Coriolus versicolor. Holzforschung, 41, 67–71.
Hoch, H.C. (1986). Freeze-substitution of fungi. In: Ultrastructural Techniques for Microorganisms, pp. 183–212, (Aldrich, H.C., and W.J. Todd, Eds.), Plenum Press, NY.
Honegger, R. (1985). Scanning electron microscopy of the fungus-plant cell interface: A simple preparative technique. Trans. Brit. Mycol. Soc., 84, 530–533.
Kates, K. (1986). Techniques of lipidology-isolation, analysis and identification of lipids. Chapter 3-Lipid extraction procedures, In: Laboratory Techniques in Biochemistry and Molecular Biology v. 3, part 2, pp. 1–467. (R.H. Burden and P.H. Van Knippenberg, Eds.), Elsevier, Amsterdam.
Micales, J.A., and Highley, T.L. (1990). Factors associated with decay capacity of the brown-rot fungus Postia placenta. Biodeterioration Research.3. Plenum Publishing Corp., NY.
Nilsson, T., Daniel, G., Kirk, T.K., and Obst, J.R. (1989). Chemistry and microscopy of wood decay by some higher Ascomycetes. Holzforschung, 43, 11–18.
Palmer, J.G., Murmanis, L.L., and Highley, T.L. (1983). Visualization of hyphal sheath in wood-decay hymenomycetes. II. White-rotters. Mycologia, 75, 1005–1010.
Razin, S. (1969). The mycoplasma membrane, In: The Mycoplasmatales and the L-phase of bacteria, pp. 317–348. (L. Hayflick, Ed.), Appleton-Century-Crofts, N.Y.
Read, N.D., Porter, R., and Beckett, A. (1982). A comparison of preparative techniques for the examination of the external morphology of fungal material with the scanning electron microscope. Can. J. Bot., 61, 2059–2078.
Sleytr, V.B., and Robard, A.W. (1982). Understanding the artifact problem in freeze fracture replication: A review. J. Microscopy, 126, 101–122.
Todd, W.J. (1986). Effects of specimen preparation on the apparent ultrastructure of microorganisms, In: Ultrastructure Techniques for Microorganisms, pp. 87-100.
Tsuneda, A., Koshitani, H., and Furukawa, I. (1987). Micromorphological patterns of incipient wood decay by Lentinus edodes. Rept. Tottori Mycol. Inst., 25, 36–48.
Waterman, R.E. (1982). Preparation of embryonic tissues for SEM. Scann. Elect. Micr., 2, 21–44.
Wilcox, W.W., and Brier, Allison. (1987). Fixation improves image of fungal hyphae in SEM. IAWA Bull., (n.s.), 8, 78–79.
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Green, F., Larsen, M., Highley, T. (1990). Ultrastructural Morphology of the Hyphal Sheath of Wood Decay Fungi Modified by Preparation for Scanning Electron Microscopy. In: Llewellyn, G.C., O’Rear, C.E. (eds) Biodeterioration Research. Biodeterioration Research, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9453-3_25
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