Mycotoxicoses, In Vitro and In Vivo: Conjecture-Hypothesis-Validation

  • Ronald T. Riley
Part of the Biodeterioration Research book series (BIOR, volume 3)


The ultimate goal of food safety research is to ensure the quality and safety of the food supply. This includes the safety of both animal feeds and human foods. Within the concept of “biodeterioration”, the impairment of human and animal health through the consumption of fungal metabolites in the food supply seems an appropriate, if not the ultimate, subject for study. Our health, and that of the animals upon which we depend, is clearly an area of great public concern. This concern creates the social and political force which sustains the monetary support of all professional scientists who study the biological activity of fungal metabolites which contaminate foods and feeds. Unfortunately, the public’s concern for food and feed safety is sometimes forgotten or avoided by scientists who enjoy doing experimental research. Few would disagree, that solving basic problems is important, however, the consumer’s concern is the applied problem -food and feed safety. In the field of mycotoxicology, it is difficult to maintain focus on this applied problem because the chemical and biological diversity of the subject matter reveals an infinite number of exciting and rewarding areas for basic research, while the epidemiological database that supports the hypothesis that fungal toxins in the food supply are a threat to American consumers is extremely limited or non-existent (Food and Chemical News, 1986).


Fungal Metabolite Fusarium Moniliforme Cyclopiazonic Acid Material Safety Data Sheet Penicillic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ames, B.N and Gold, L.S. (1989). Pesticides, risk, and applesauce. Science, 244, 755–757.CrossRefGoogle Scholar
  2. Bezuidenhout, S.C., Gelderblom, W.C.A., Gorst-Allman, C.P., Horak, R.M., Marasas, W.F.O., Spiteller, G. and Vleggaar, R. (1988). Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J. Chem. Soc., Chem. Commun. 743.Google Scholar
  3. Food and Chemical News (1986). Mycotoxins rarely affect humans, IFT concludes. Food and Chemical News, Inc, Washington, DC, Vol. 28(11), 44.Google Scholar
  4. Food and Chemical News (1989a). House panel assured aflatoxin problem in corn under control. Food and Chemical News, Inc, Washington, DC, Vol. 31(6), 44–46.Google Scholar
  5. Food and Chemical News (1989b). NTP considering listing BHA in carcinogen report. Food and Chemical News, Inc, Washington, DC, Vol. 31(1), 61–62.Google Scholar
  6. Gelderblom, W.C.A., Thiel, P.G., Van der Merwe, K.J., Marasas, W.F.O. and Spies, H.S.C. (1983). A mutagen produced by Fusarium moniliforme. Toxicon, 21, 467–473.CrossRefGoogle Scholar
  7. Gelderblom, W.C.A., Thiel, P.G., Jaskiewicz, K., and Marasas, W.F.O. (1986). Investigations on the carcinogenicity of fusarin C-a mutagenic metabolite of Fusarium moniliforme. Carcinogenesis, 7, 1899–1901.CrossRefGoogle Scholar
  8. Gelderblom, W.C.A., Thiel, P.G., Jaskiewicz, K., Horak, R.M., Marasas, W.F.O., Vleggaar, R. and Kriek, N.P.J. (1988a). The fumonisins-novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Appl. Environ. Microbiol., 54, 1806–1811.Google Scholar
  9. Gelderblom, W.C.A., Marasas, W.F.O., Jaskiewicz, K., Combrinck, S. and van Schalkwyk, D.J. (1988b). Cancer promoting potential of different strains of Fusarium moniliforme in a short-term cancer initiation/promotion assay. Carcinogenesis, 9, 1405–1409.CrossRefGoogle Scholar
  10. Goeger, D. E., Riley, R. T., Dorner, J. W. and Cole R. J. (1988). Cyclopiazonic acid inhibition of the Ca+2-transport ATPase in rat skeletal muscle sarcoplasmic reticulum vesicles. Biochem. Pharmac., 37, 978–981. 1988.CrossRefGoogle Scholar
  11. Goeger, D. E. and Riley, R. T. (1989). Interaction of cyclopiazonic acid with rat skeletal muscle sarcoplasmic reticulum vesicles; effect on Ca+2 binding and Ca+2 permeability. Biochem. Pharmac., (in press).Google Scholar
  12. Greenberg, R.A. (1989). Food safety workshop report gets widespread dissemination. Fd. Technol., 43, 26–29.Google Scholar
  13. Groth, E. (1989). Alar in apples. Science, 244, 755.CrossRefGoogle Scholar
  14. International Agency for Research on Cancer (1986). IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans; Some Naturally Occurring and Synthetic Food Components, Furocoumarins and Ultraviolet Radiation, Vol. 40, pp. 83–98, IARC, Lyon, France.Google Scholar
  15. International Agency for Research on Cancer (1987). IARC Monographs on the Evaluation of the Carcinogenic Risks to Humans-Overall Evaluation of Carcinogenity: an Updating of IARC Monographs Volumes 1 to 42, Supplement 7, pp. 83-87, IARC, Lyon, France.Google Scholar
  16. Jelinek, C.F., Pohland, A.E. and Wood, G.E. (1989). Worldwide occurrence of mycotoxins in food and feeds—an update. J._Assoc. Off. Anal. Chem., 72, 223–230.Google Scholar
  17. Kilman, S. (1989). Spreading poison. Wall Street Journal, 23 February: Al.Google Scholar
  18. Komulainen, H. and Bondy, S.C. (1988). Increased free intracellular Ca2+ by toxic agents: an index of potential neurotoxicity. TIPS, 9, 154–156.Google Scholar
  19. Koshland, D.E. (1989). Scare of the week. Science, 244, 9.CrossRefGoogle Scholar
  20. Krueger, B.K. (1989). Toward an understanding of structure and function of ion channels. FASEB J., 3, 1906–1914.Google Scholar
  21. Marasas, W.F.O., Nelson, P.E. and Toussoun, T.A. (1984). Toxigenic Fusarium Species. pp. 216–252, The Pennsylvania State University Press, University Park, PA.Google Scholar
  22. Marasas, W.F.O., Kellerman, T.S., Gelderblom, W.C.A., Coetzer, J.A.W., Thiel, P.G. and van der Lugt, J.J. (1988a). Leucoencephalomalacia in a horse induced by Fumonisin B1 isolated from Fusarium moniliforme. Onderstepoort. J. vet. Res., 55, 197–203.Google Scholar
  23. Marasas, W.F.O., Jaskiewicz, K., Venter, F.S. and van Schalkwyk, D.J. (1988b). Fusarium moniliforme contamination of maize in oesophageal cancer areas in Transkei. S. Afr. Med. J., 74, 110–114.Google Scholar
  24. Marshall, E. (1983). Yellow rain experts battle over corn mold. Science, 221, 526–529.CrossRefGoogle Scholar
  25. Morrissey, R.E., Norred, W.P., Cole, R.J. and Dorner, J. (1985). Toxicity of the mycotoxin, cyclopiazonic acid, to Sprague-Dawley rats. Toxicol. Appl. Pharmacol., 77, 94–107.CrossRefGoogle Scholar
  26. Peden, M.W. (1989). Effects of cyclopiazonic acid: Guinea pig skeletal muscle, In: Cellular and molecular mode of action of selected microbial toxins in foods and feeds, (A. Pohland ed.), Plenum Publishing Corp., New York, NY (in press).Google Scholar
  27. Purchase, I.F.H. (1971). The acute toxicity of the mycotoxin cyclopiazonic acid to rats. Toxicol. Appl. Pharmacol., 18, 114–123.CrossRefGoogle Scholar
  28. Rao, B.L. and Husain, A. (1985). Presence of cyclopiazonic acid in kodo millet (Paspalum scrobiculatum) causing ‘kodua poisoning’ in man and its production by associated fungi. Mycopathologia, 89, 177–180.CrossRefGoogle Scholar
  29. van Rensburg, S.J. (1984). Subacute toxicity of the mycotoxin cyclopiazonic acid. Fd. Chem. Toxicol., 22, 993–998.CrossRefGoogle Scholar
  30. Riley, R. T., Showker, J. L., Cole, R. J. and Dorner, J. W. (1986). The mechanism by which cyclopiazonic acid potentiates accumulation of tetraphenylphosphonium by cultured renal epithelial cells. J. Biochem. Toxicol., 1(4), 13–29.CrossRefGoogle Scholar
  31. Riley, R. T., Goeger, D. E., Norred, W. P., Cole R. J. and Dorner, J. W. (1987). Age and growth related changes in cyclopiazonic acid-potentiated lipophilic cation accumulation by cultured cells and binding to freeze-thaw lysed cells. J. Biochem. Toxicol., 2(4), 251–264.CrossRefGoogle Scholar
  32. Riley, R. T., Goeger, D. E. and Hinton, D.M. (1989). Mycotoxin-induced alterations in ion transport across cell membranes, In: Cellular and molecular mode of action of selected microbial toxins in foods and feeds, (A. Pohland ed.), Plenum Publishing Corp., New York, NY (in press).Google Scholar
  33. Scheuplein, R.J. (1990). Perspectives on toxicological risk: An example: food-borne carcinogenic risk, In: Progress in predictive toxicology, (I.C. Munro, T. Chubick and J.A. Swenburg, eds.), Elsevier Press, Amsterdam (in press).Google Scholar
  34. Trump, B.F., Berezesky, I.K. and Phelps, P.C. (1981). Sodium and calcium regulation and the role of the cytoskeleton in the pathogenesis of disease: a review and hypothesis. Scanning Electron Microsc. II, 435–454.Google Scholar
  35. Voss, K.A., Norred, W.P., Plattner, R.D. and Bacon, C.W. (1989). Hepatotoxicity and renal toxicity in rats of corn samples associated with field cases of equine leucoencephalomalacia. Fd. Chem. Toxicol., 27, 89–96.CrossRefGoogle Scholar
  36. Voss, K.A., Norred, W.P., Hinton, D.M., Cole, R.J. and Dorner, J. (1989). Subchronic oral toxicity of cyclopiazonic acid (CPA) in male Sprague-Dawley rats. Mycopathologia (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Ronald T. Riley
    • 1
  1. 1.Toxicology and Mycotoxins Research Unit, R.B. Russell Research CenterUSDA-ARSAthensUSA

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