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Polymerase Chain Reaction Analysis, Detection and Typing of Toxin Producing Clostridium Difficile

  • David E. McMillin
  • Lycurgus L. Muldrow
Part of the Biodeterioration Research book series (BIOR, volume 4)

Abstract

Clostridium difficile, the primary cause of pseudomembranous colitis (PMC) in humans, produces two toxins; toxin A, an hemorrhagic enterotoxin and toxin B, a potent cytotoxin. Both toxin proteins appear to act synergistically to induce colitis and PMC. Size estimates of the toxin proteins have varied widely; however recent DNA sequence information estimated the size of toxin A to be 307,972 daltons (Dove et al. 1990 4 and toxin B to be 269,696 daltons (Barroso et al. 1990). These large bio-toxins are only found in pathogenic strains of C. difficile but not found in non-pathogenic strains. In addition to the studies of the toxin proteins, a number of labs have been investigating the molecular biology of these toxin genes.

Keywords

Clostridium Difficile Toxin Gene Pseudomembranous Colitis Toxin Protein Polymerase Chain Reaction Procedure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • David E. McMillin
    • 1
  • Lycurgus L. Muldrow
    • 1
  1. 1.Department of BiologyClark Atlanta UniversityAtlantaUSA

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