Protein Contents of Ozone- and Air-Fumigated Pinus Strobus Needles

  • Eunice T. Dlamini
  • Arthur L. Williams
  • William V. Dashek
  • Wayne T. Swank
  • James M. Vose
Part of the Biodeterioration Research book series (BIOR, volume 4)

Abstract

Ozone (O3) is normally present within the earth’s troposphere at 20–30ppb concentration. However, as a result of increased industrialization and urbanization, e.g., automobile emissions (Elstner, 1984), O3 has become an undesirable atmospheric pollutant in many densely-populated areas of the United States. However, monitoring of atmospheric O3 at the Coweeta Hydrologic Laboratory (Otto, NC) during 1988 revealed April through September fluctuations of 40–70 ppb (Figure 1), and mean hourly concentrations sometimes exceed 90 ppb. Thus, even rural areas experience elevated atmospheric O3 levels, presumably as a result of long-range transport of ozone precursors (e.g., NOx).

Keywords

Total Protein Content Ozone Precursor Hourly Concentration Automobile Emission Eastern White 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barnes, R.L. (1972). Effects of chronic exposure to ozone on photosynthesis and respiration of pines. Environ. Pollut. 3, 133–138.CrossRefGoogle Scholar
  2. Berry, C.R. (1973). Age of pine seedlings with primary needles affects sensitivity to ozone and sulfur dioxide. Phvtopathol. 64, 207–209.CrossRefGoogle Scholar
  3. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248.CrossRefGoogle Scholar
  4. Chevone, B.I. and Linzon, S.M. (1988). Tree decline in North America. Environ. Pollut. 50, 876–99.CrossRefGoogle Scholar
  5. Dashek, W.V. and Erickson, S.S. (1981). Isolation, assay, biosynthesis, metabolism, uptake and translocation and functions of proline in plant cells and tissues. Bot. Review. 47, 340–385.CrossRefGoogle Scholar
  6. Dashek, W.V., Williams A.L., and Zeidan, H., (1990). Needle peroxidase, a potential monitor for ozone-induced damage to eastern white pine seedlings. Plant Physiol. 93, 156.Google Scholar
  7. Durzan, D.J. and Stewart, F.C. (1967). The nitrogen metabolism of Picea glauca (Mensch) Voss and Pinus bankstrana lamb as influenced by mineral nutrition. Can. J. Bot. 45, 695–710.CrossRefGoogle Scholar
  8. Elstner, E.F. (1984). Comparison of “Inflammation” in pine needles and humans. In: Oxygen radicals in Chemistry and Biology. ( Bos W., Sara, M., and Tait, D., eds.) Walter Dehydryterate Co., Berlin.Google Scholar
  9. Elzey, F.F. (1987). Introductory Statistics: A microcomputer approach. Brooks/Cole Publishing Company, Monterey, CA.Google Scholar
  10. Erickson, S.S. and Dashek, W.V. (1982). Accumulation of foliar soluble proline following fumigation of Glycine max, cv. “Essex” and “Excelsior” seedlings with sulphur dioxide. Environ. Pollut. 28, 89–108.CrossRefGoogle Scholar
  11. Hogsett, W.E., Plocker, M., Wildman, V., Tingey D.T., and Bennett, J.P. (1985). Growth response of two varieties of slash pine seedlings to chronic ozone exposures. Can. J. Bot. 63, 2369–2376.CrossRefGoogle Scholar
  12. Kozlowski, T.T. (1980). Impacts of air pollution on forest ecosystems. American Institute of Biological Sciences, WI.Google Scholar
  13. Kress, L.W. and Skelly, J.M. (1982). Response of several-forest tree species to chronic doses of ozone and nitrogen. Plant Dis. 66, 1149–1152.CrossRefGoogle Scholar
  14. Laemmli, V.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227, 680–685.CrossRefGoogle Scholar
  15. Maniatis, T., Fritsch, E.F., and Sambrock, J. (1982). Molecular Cloning, A laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  16. Miller, P.R. and Stottle, K.W. (1984). Response of forest species to 03, SO2 + NO2 mixtures. Proceedings APCA Annual Meeting 77, 84–305.Google Scholar
  17. Peterson, D.L., Arbaugh, M.J., Wakefield, V.A., and Miller, P.R. (1987). Evidence of growth reduction in ozone-injured Jeffrey pine (Pinus jeffreyi, Grey. and Balf.) in Sequoia and Kings Canyon National Parks. J Air Pollut. Control Assoc. 37, 906–912.Google Scholar
  18. Pye, J.M. (1988). Impact of ozone on the growth and yield of trees: A review. J. Environ. Qual. 17, 347–360.CrossRefGoogle Scholar
  19. Smith, W.H. (1981). Air Pollution and Forests. Interaction Between Air Contaminants and Forest Ecosystems. Springer-Verlag, New York.Google Scholar
  20. Stewart, C.R. (1972). Effects of proline and carbohydrates on the metabolism of exogenous proline by excised bean leaves in the dark. Plant Phvsiol. 50, 551–555.CrossRefGoogle Scholar
  21. Swank, W.T. and Vose, J.M. Watershed-scale response to some events in a Pinus strobus L. plantation. Water, Air and Soil Pollution (in press).Google Scholar
  22. Taylor, O.C. (1984). Organismal responses of higher plants to atmospheric pollutants: Photochemical and Other. In: Air Pollution and Plant Life ( M. Treshow, ed.). John Wiley and Sons, Ltd. pp. 215–223.Google Scholar
  23. Wilkinson, T.G. and Barnes, R.L. (1972). Effects of ozone on 14CO2 fixation patterns in pine. Can. J. Bot. 51, 1573–1578.CrossRefGoogle Scholar
  24. Wu, R., Grassman, L., and Moldave, K. (1983). Recombinant DNA, parts B and C, Academic Press, NY.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Eunice T. Dlamini
    • 1
  • Arthur L. Williams
    • 1
  • William V. Dashek
    • 1
  • Wayne T. Swank
    • 2
  • James M. Vose
    • 2
  1. 1.Department of BiologyClark Atlanta UniversityAtlantaUSA
  2. 2.Coweeta Hydrologic LaboratoryUnited States Department of AgricultureOttoUSA

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