Distribution of Polyphenol Oxidase in Organelles of Hyphae of the Wood-Deteriorating Fungus, Coriolus Versicolor

  • Nina L. Moore
  • L. A. Brako
  • W. V. Dashek
  • C. A. Clausen
  • B. R. Jones
Part of the Biodeterioration Research book series (BIOR, volume 4)

Abstract

Coriolus versicolor, a white-rot Basidiomycete, secretes cellulolytic and ligninolytic enzymes (Higuchi, 1953; Rosenberg, 1979; Harvey et al., 1987) as well as polyphenol oxidase (Taylor et al., 1987; Moore et al., 1989). Whereas the former degrade certain wood polymers (Higuchi, 1985), the latter can convert o-diphenols to o-diquinones (Fric, 1976) and oligomerize syringic acid (Liu et al., 1981), a lignin derivative. In this connection, certain phenolic compounds can serve as disease-resistance factors possibly regulating the proliferation of wood-decay fungi within host tissues (Shigo, 1984; Dashek et al., 1990). Because ligno-cellulolytic enzymes are of commercial value to the paper-pulp industry (Zadrazil and Reiniger, 1988) and the agricultural community (Van der Meer et al., 1987), an inexpensive and “readily-available” supply of these enzymes is highly desirable. As C. versicolor can be “batch-cultured”, over-production and enhanced secretion of these enzymes are feasible (Fahraeus and Reinhammer, 1967). While over-production could possibly be achieved by substrate induction and/or recombinant DNA technologies (Maniatis et al., 1982), secretion might be enhanced by controlling cellular energy gradients and/or possible glycosylation of PPO.

Keywords

Cacodylate Buffer Polyphenol Oxidase Lignocellulolytic Enzyme Coriolus Versicolor Lignin Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Nina L. Moore
    • 1
  • L. A. Brako
    • 1
  • W. V. Dashek
    • 1
  • C. A. Clausen
    • 2
  • B. R. Jones
    • 3
  1. 1.Department of BiologyClark Atlanta UniversityAtlantaUSA
  2. 2.USDA Forest ServiceForest Products LaboratoryMadisonUSA
  3. 3.Department of BiologyMorris Brown CollegeAtlantaUSA

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