Stimulation of Zearalenone Production in Rice Cultures of Fusarium Semitectum by Addition of Zearalenone and α-and β-Zearalenol

  • K. E. Richardson
  • W. M. HaglerJr
  • P. B. Hamilton
Part of the Biodeterioration Research book series (BIOR, volume 4)


Zearalenone is an estrogen produced by several species of Fusarium on corn and other cereal grains (Caldwell and Tuite, 1974; Gross and Robb, 1975; Bottalico, 1979). Its presence in animal feeds and subsequent consumption have been associated with hyperestrogenism and other reproductive disorders in swine (Miller et al., 1973), poultry (Meronuck et al., 1970; Allen et al., 1981a,b) and cattle (Mirocha et al., 1968). Zearalenol, which is produced in its two epimeric forms as a metabolic precursor of zearalenone by certain species of Fusarium exhibits estrogenic activity (Peters, 1972; Mirocha et al., 1978; Bottalico et al., 1985; Richardson et al., 1985). α-Zearalenol has three to four times as much estrogenic activity as zearalenone while β-zearalenol has about the same or slightly less estrogenicity as zearalenone (Peters, 1972; Mirocha et al., 1978; Hagler et al., 1979). Zearalenone and zearalenol also have some anabolic activity that is useful in animal nutrition (Hidy et al., 1977).


High Pressure Liquid Chromatography Estrogenic Activity Catalytic Amount Rice Culture Fusarium Species 
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  1. Allen, N.K., Mirocha, C.J., Aakhus-Allen, S., Bitgood, J.J., Weaver, G., and Bates, F. (1981). Effect of dietary zearalenone on reproduction in chickens. Poultry Sci. 60, 1165–1174.CrossRefGoogle Scholar
  2. Allen, N.K., Mirocha, C.J., Weaver, G., Aakhus-Allen, S., and Bates, F. (1981). Effects of dietary zearalenone on finishing broiler chickens and young turkey poults. Poultry Sci. 60, 124–131.Google Scholar
  3. Bacon, C.W., Robbins, J.D., and Barter, J.K. (1977). Media for identification of Gibberella zeae and production of F-2 (zearalenone). Appl. Environ. Microbiol. 33, 445–449.Google Scholar
  4. Bottalalico, A. (1979). On the occurrence of zearalenone in Italy. Mycopathologia 67, 119–121.Google Scholar
  5. Bottalico, A., Visconti, A., Logrieco, A., Solfrizzo, M., and Mirocha, C.J. (1985). Occurrence of zearalenols (diastereomeric mixture) in corn stalk rot and their production by associated Fusarium species. Appl. Environ. Microbiol. 49, 547–551.Google Scholar
  6. Caldwell, R.W. and Tuite, J. (1974). Zearalenone in freshly harvested corn. Phvtopathology 64, 752–753.Google Scholar
  7. Eugenio, C.P., Christensen, C.M., and Mirocha, C.J. (1970). Factors affecting production of the mycotoxin F-2 by Fusarium roseum. Phytopathologv 60, 1055–1057.Google Scholar
  8. Gross, V.J. and Robb, J. (1975). Zearalenone production in barley. Ann. Appl. Biol., 80, 211–216.Google Scholar
  9. Hagler, W.M., Jr., Mirocha, C.J., Pathre, S.V., and Behrens, J.C. (1979). Identification of the naturally occurring isomer of zearalenol produced by Fusarium roseum ‘Gibbosum’ in rice culture. Appl. Environ. Microbiol. 37, 848–853.Google Scholar
  10. Hagler, W.M., Jr. and Mirocha, C.J. (1980). Biosynthesis of [14C] zearalenone from [1-14C]-acetate by Fusarium roseum ‘Gibbosum’. Appl. Environ. Microbiol. 39, 668–670.Google Scholar
  11. Hidy, P.H., Baldwin, R.S., Gresham, R.L., Keith, C.L., and McMullen, J.R. (1977). Zearalenone and some derivatives: production and biological activities. Adv. Appl. Microbiol. 22, 59–82.Google Scholar
  12. Marasas, W.F.O., Nelson, P.E., and Toussoun, T.A. (1984). Toxigenic FUSARIUM Species: Identity and Mycotoxicology. pp. 99–110, The Pennsylvania State University Press, College Park, PA.Google Scholar
  13. Meronuck, R.A., Garren, K.H., Christensen, C.M., Nelson, G.H., and Bates, F. (1970). Effects on turkey poults and chicks of rations containing corn invaded by Penicillium and Fusarium species. Am. J. Vet. Res. 31, 551555.Google Scholar
  14. Miller, J.K., Hacking, A., and Gross, V.J. (1973). Stillbirths, neonatal mortality and small litters in pigs associated with the ingestion of Fusarium toxins by pregnant sows. Vet. Rec. 93, 555–559.Google Scholar
  15. Mirocha, C.J., Harrison, J., Nichols, A.A., and McClintock, M. (1968). Detection of a fungal estrogen (F-2) in hay associated with infertility in dairy cattle. Appl. Microbiol. 16, 797–798.Google Scholar
  16. Mirocha, C.J., Pathre, S.V., Behrens, J., and Schauerhamer, B. (1978). Uterotropic activity of cis and trans isomers of zearalenone and zearalenol. Aool. Environ. Microbiol. 35, 986–987.Google Scholar
  17. Peters, C.A. (1972). Photochemistry of zearalenone and its derivatives. J. Med. Chem. 15, 867–868.Google Scholar
  18. Richardson, K.E., Hagler, Jr., W.M., and Hamilton, P.B. (1984). Bioconversion of a-[14C] zearalenol and ß-[14C] zearalenol into [14C] zearalenone by Fusarium roseum ‘Gibbosum’. Apol. Environ. Microbiol. 47, 1206–1209.Google Scholar
  19. Richardson, K.E., Hagler, Jr., W.M., and Mirocha, C.J. (1985). Production of zearalenone, a-and ß-zearalenol by Fusarium spp. in rice culture. J. Agric. Food Chem. 33, 862–866.Google Scholar
  20. Sarudi, I. (1976). Hydrogenation used in the detection of zearalenone (F-2 toxin). Acta Aliment. 5, 37–40.Google Scholar
  21. SAS. (1982). SAS Users Guide. Statistics. Statistical Analysis System Institute, Inc., Cary, NC. Shipchandler, M.T. (1975). Chemistry of zearalenone and some of its derivatives. Heterocycles 3, 471–520.Google Scholar
  22. Steele, J.A., Mirocha, C.J., and Pathre, S.V. (1976). Metabolism of zearalenone by Fusarium roseum ‘Graminearum’. Mycopathologia 57, 77–78.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • K. E. Richardson
    • 1
  • W. M. HaglerJr
    • 2
  • P. B. Hamilton
    • 1
  1. 1.Department of Poultry ScienceNorth Carolina State UniversityRaleighUSA
  2. 2.Mycotoxin Laboratory, Department of Poultry ScienceNorth Carolina State UniversityRaleighUSA

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