Potential of Toluene-Degrading Systems for the Construction of Hybrid Pathways for Nitrotoluene Metabolism
Wastes from manufacturing processes, together with the widespread use of some nitroaromatic compounds, have polluted waters and soils. Because of their toxicity and mutagenicity, some of these compounds have been designated pollutants whose elimination is a high priority (25). A variety of alternative treatments including physical, chemical and biological (21), have been developed to reduce or eliminate contamination due to hazardous nitroorganic compounds. Physical treatments comprise absorption to activated carbon, filtration and incineration. Chemical approaches involve solvent extraction or surfactant precipitation. These treatments are expensive, and may in some cases generate unwanted products (22). The biological approach, including continuous and batch treatments of liquid wastes, and composting and soil treatments, is usually cheaper and involves less risk to human health or the environment (12, 23, 54). However, nitroaromatic compounds are relatively refractory to mineralization by microbes.
KeywordsNitro Group Benzyl Alcohol Pseudomonas Putida Catabolic Pathway Sole Nitrogen Source
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- 15.Haïdour, A., and J. L. Ramos. Identification of products resulting from the biological reduction of 2,4,6-trinitrotoluene, and 2,4-and 2,6-dinitrotoluene by Pseudomonas sp. Appl. Environ. Microbiol., submitted.Google Scholar
- 17.Haigier, B.E., W.H. Wallace, and J.C. Spain. 1994. Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42. Appl. Environ. Microbiol. 60:3466–3469.Google Scholar
- 18.Harayama, S., R. A. Leppik, M. Rekik, N. Mermod, P. R. Lehrbach, W. Reinecke, and K. N. Timmis. 1986. Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product. J. Bacteriol. 187:455–461.Google Scholar
- 21.Kaplan, D.L. 1989. Biotransformation pathways of hazardous energetic organo-nitro compounds, p 155–181.In D. Kamely, A. Chakrabarty, and G. Omenn (ed.), Biotechnology and biodegradation. Portfolio Publishing Co, Houston.Google Scholar
- 33.Nadeau, L. J., and J. Spain. 1994. The bacterial degradation of m-nitrobenzoic acid, abstr. Q-114, p. 408. Abstr. 94th Annu. Meet. Amer. Soc. Microbiol. 1994.Google Scholar
- 57.Wubbolts, M. G. 1994. Xylene and alkane mono-oxygenases from Pseudomonas putida. Ph.D. dissertation. University of Groningen, Groningen, The Netherlands.Google Scholar