Analysis of the Actions of Anti-Inflammatory Drugs, with Particular Reference to Effects on Interleukins and Leucocytes

  • K. D. Rainsford
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

The importance of IL’s* and other cytokines in chronic inflammation has aroused interest in their assay in contexts such as inflammatory exudates and stimulated or unstimulated leucocytes. Assays developed for two main IL’s, viz. IL-1 and IL-2, include cell responses such as production of other mediators, or reactions (e.g. proliferation, aggregation) whose specificity depends on the responsiveness of the cell type as well as the purity of the extracts, fluids or culture media being assayed, and on the assay conditions. Certain cell clones are more selective in the response to the IL’s (e.g. the NOB-1 sub-clone of the EL-4 cell for IL-1), so improving the specificity of the LAF assay. Conditions are described for improving the specificity and responses of IL-1 in the CRA using the bovine nasal septum. The recent development of RIA’s and ELISA’s for assaying IL-1 and IL-2 is considered together with limitations and conditions in applying the methods. Appropriate animal models for studies of the effects of anti-rheumatic agents are discussed in relation to the use of the above-mentioned assays.

Keywords

Proteoglycan Degradation Bovine Nasal Cartilage Bovine Nasal Septum Strangeways Research Laboratory Polymorphonuclear Neutrophil Leucocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

HETES

hydroxyeicosatetraenoic acids

IL

interleukin

LA(F)

lymphocyte activation (factor)

LT

leukotriene

(NS)AID

(non-steroidal) anti-inflammatory drug

PG

prostaglandin

PMN

polymorphonuclear neutrophil leucocyte

CRA

see text

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • K. D. Rainsford
    • 1
  1. 1.Anti-inflammatory Research UnitStrangeway’s Research LaboratoryCambridgeUK

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