Design of and First Experiments with a Bioluminescence Detector for HPLC

  • H. M. Ruijten
  • B. E. Timmerman
  • H. de Bree
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

As discussed earlier in this book [Brinkman, #F-2; de Bree, #NC(E)-4], HPLC still lacks a universal sensitive and selective detector, and for biomedical and environmental trace-level analyses HPLC is still outclassed by GC (especially capillary with the new chemically bonded phases). The efficacy of enzymes in metabolizing drugs should be exploitable to furnish a selective post-column reactor for HPLC, since mostly the selective enzymatic conversions involve production of NAD, ATP or H2O2. Several bioluminescence kits based on light emission initiated by one of these products are now commercially available. Amongst the many bioluminescence procedures found in the literature, most are in the food and clinical chemistry areas and none are HPLC-linked. In the approach now described, both the coenzyme-generating enzymes and those that emit light are immobilized, which entails problems that are now considered along with prospects for the detection approach in general. At the outset our model compound was flesinoxan

Keywords

Model Compound Light Emission Detection Approach Reaction Light Alcohol Oxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Krämer, D.M., Lehmann, K., Pennewiss, H. & Plainer, H. (1975) in 23rd Colloquium, Protides of Biological Fluids (Brugge, 1975), Pergamon, Oxford, pp. 505–512.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • H. M. Ruijten
    • 1
  • B. E. Timmerman
    • 1
  • H. de Bree
    • 1
  1. 1.Duphar Research LaboratoriesWeespThe Netherlands

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