Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular pp 389-392 | Cite as
NMR as an Aid in Studying N-Alkylformamides and Metabolites
Abstract
The common industrial and laboratory solvent DMF≠ and its close analogue NMF share the ability to cause hepatotoxicity in man. They differ, however, in their biological properties towards rodents. NMF is a hepatotoxin towards rats and mice and shows significant activity against tumours of murine [1] and human [2] origin grown in mice. DMF shows neither strong therapeutic nor hepatotoxic activity in mice, whereas NEF in mice shows hepatoxicity only [1, 3]. The explanation for these comparisons and contrasts may be connected with the nature and quantity of the metabolites formed from different N-alkylformamides in the various species. The characterization of the urinary metabolites by conventional techniques such as MS and UV spectrometry is hampered by their lability and lack of a suitable chromophore.
Keywords
Urinary Metabolite Free Induction Decay Mercapturic Acid Developmental Therapeutics Program Cancer Research CampaignAbbreviation
- N,N-dimethyl
DMF
- N-methyl
NMF
- N-ethyl
NEF; see over for HMMF
- N-acetyl-S-(N-methylcarbamoyl)cysteine
AMCC
- if Et
not Me: AECC
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