Proton NMR Studies on the Metabolism and Biochemical Effects of Hydrazine In Vivo
High-resolution 1H NMR spectroscopy is valuable in the bioanalysis of biological fluids (e.g. [1–6], and Vols. 16 & 17 in this series — J.K. Nicholson). Notably, it allows quantitative information on a wide range of endogenous and xenobiotic metabolites to be collected simultaneously. As NMR is non-selective in the detection of metabolites (all compounds present at >0.1 mM levels being detectable), it is particularly appropriate for the investigation of the metabolism of compounds with poorly understood metabolic profiles.
KeywordsAliphatic Region Field Frequency Lock Xenobiotic Metabolite Hydrazine Metabolite 3Central Toxicology Laboratory
free induction decay [cf. GC use of ‘FID’-Ed,]
1,4,5,6-tetrahydro-6-oxo-3-pyridazine carboxylic acid
Unable to display preview. Download preview PDF.
- 1.Nicholson, J.K., Timbrell, J.A., Bales, J.R. & Sadler, P.J. (1985) Mol. Pharmacol. 27, 634–643.Google Scholar
- 3.Bales, J.R., Nicholson, J.K. & Sadler, P.J. (1986) Clin. Chem. 31, 757–762.Google Scholar
- 5.Tulip, K., Timbrell, J.A., Wilson, I.D., Troke, J. & Nicholson, J.K. (1986) Drug Met. Disp. 14, 746–749.Google Scholar
- 9.Cohen, S.M. (1983) J. Biol. Chem. 258, 14294–14308.Google Scholar
- 10.Cohen, S.M. (1984) Fed. Proc. 43, 2657–2662.Google Scholar