Identification of a New Metabolite of Leukotriene B4 by RP-HPLC and GC-MS Analysis
RP-HPLC with UV detection is the method of choice for rapid identification and quantification of LT’s*. The large number of structurally related compounds precludes separating all of them within one system. Therefore RP-HPLC systems have been developed to isolate particular groups of compounds such as peptidoLT’s or LTB4 and its isomers. A RP-HPLC system now described allows the separation of a new metabolite of LTB4 from LTB4 and its trans isomers.
KeywordsTrans Isomer Tetraenoic Acid Lysosomal Enzyme Release Cultivate Mesangium Cell Human Inflammatory Cell
Unable to display preview. Download preview PDF.