The Structure Elucidation of Drug Metabolites Using Thermospray LC-MS and LC-MS-MS

  • T. J. A. Blake
  • I. G. Beattie
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


The isolation of individual metabolites is often laborious and time-consuming, yet in the past was mandatory if structural characterization was required. The advent of directly coupled liquid chromatography [HPLC]-mass spectrometry (LC-MS) has greatly facilitated structure determination of drug metabolites without the need for isolation. This article concerns the thermospray interface, which has proved to be very reliable, and the combined potential of LC-MS and LC-MS-MS as highly specific identification techniques, along with collisionally activated decomposition, as shown for temelastine.


Drug Metabolite Faecal Extract Collisionally Activate Decomposition Authentic Reference Compound Combine Potential 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • T. J. A. Blake
    • 1
  • I. G. Beattie
    • 1
  1. 1.Department of Drug MetabolismSmith Kline & French Research Ltd.The Frythe, Welwyn, HertsUK

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