Abstract
The isolation of individual metabolites is often laborious and time-consuming, yet in the past was mandatory if structural characterization was required. The advent of directly coupled liquid chromatography [HPLC]-mass spectrometry (LC-MS) has greatly facilitated structure determination of drug metabolites without the need for isolation. This article concerns the thermospray interface, which has proved to be very reliable, and the combined potential of LC-MS and LC-MS-MS as highly specific identification techniques, along with collisionally activated decomposition, as shown for temelastine.
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References
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© 1988 Springer Science+Business Media New York
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Blake, T.J.A., Beattie, I.G. (1988). The Structure Elucidation of Drug Metabolites Using Thermospray LC-MS and LC-MS-MS. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_49
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DOI: https://doi.org/10.1007/978-1-4757-9424-3_49
Publisher Name: Springer, Boston, MA
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