Retention Mechanisms in Reversed-Phase HPLC
In analytical chemistry HPLC techniques are notably powerful, important and widely used, yet they are still viewed as ‘black magic’ by many practitioners because of a lack of basic understanding of the Chromatographic process. Investigations of retention mechanisms have as their goal a complete and exact understanding of retention, accounting for influences of solute type, mobile-phase composition and stationary-phase structure. This would allow separations to be pre-calculated, largely eliminating trial-and-error development. It would also be feasible to devise a rigorously correct retention index system for HPLC, to obtain exact physicochemical information, and to develop a ‘truly expert’ system.
KeywordsTranexamic Acid Organic Modifier Chiral Separation Retention Mechanism Binary Interaction Parameter
Unable to display preview. Download preview PDF.
- 1.Edholm, L-E. (1985) Current Clin. Pract. Series 19, 238–44.Google Scholar
- 3.Weidolf, L. (1985) J. Chromatog. 343, 85–97.Google Scholar
- 5.Elmworthy, P.M., Tsementzis, S.A., Westmead, D. & Hitchcock, E.R. (1985) J. Chromatog. 343, 109–117.Google Scholar
- 6.Cooper, J.D.H. & Turnell, D.C. (1986) J. Chromatog. 380, 109–116.Google Scholar
- 10.Articles (1988) in Biochem. Pharmacol. 37 [Part 1], 1-147.Google Scholar
- 11.Cordonnier, J., Van den Heede, M. & Heyndrickx, A. (1987) Int. Analyst 1, May issue, 28–30.Google Scholar
- 12.Miller, L.J., Bouska, J.B. & Go, V.L.W. (1986) J. Chromatog. 378, 77–84.Google Scholar