Retention Mechanisms in Reversed-Phase HPLC

  • John G. Dorsey
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


In analytical chemistry HPLC techniques are notably powerful, important and widely used, yet they are still viewed as ‘black magic’ by many practitioners because of a lack of basic understanding of the Chromatographic process. Investigations of retention mechanisms have as their goal a complete and exact understanding of retention, accounting for influences of solute type, mobile-phase composition and stationary-phase structure. This would allow separations to be pre-calculated, largely eliminating trial-and-error development. It would also be feasible to devise a rigorously correct retention index system for HPLC, to obtain exact physicochemical information, and to develop a ‘truly expert’ system.


Tranexamic Acid Organic Modifier Chiral Separation Retention Mechanism Binary Interaction Parameter 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • John G. Dorsey
    • 1
  1. 1.Department of ChemistryUniversity of FloridaGainesvilleUSA

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