Solid-Phase Extraction: Personal Experiences

  • Robin Whelpton
  • Peter R. Hurst
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


The large number of types of phases and column sizes available for solid-phase extraction (SPE) makes this a very powerful and versatile sample preparation technique. As most of the available phases are based on those used in HPLC, the analyst is likely to be familiar with their behaviour. Some columns have been developed for particular applications, e.g. phenylboronic acid phases for catecholamine separation. We prefer to consider SPE as an important addition to the extraction techniques available, not necessarily as a replacement. Because of their similarities, some of the points discussed here are also applicable to column-switching techniques.


Sample Preparation Technique Column Size Trace Enrichment Fast Flow Rate Improve Quality Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Whelpton, R. & Moore, T. (1985) J. Chromatog. 341, 361–371.Google Scholar
  2. 2.
    Ruane, R.J. & Wilson, I.D. (1987) J. Pharm. Biomed. Anal. 5, 723–727.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Robin Whelpton
    • 1
  • Peter R. Hurst
    • 1
  1. 1.Department of Pharmacology and TherapeuticsLondon Hospital Medical CollegeWhitechapel, LondonUK

Personalised recommendations