An Assay for Idaverine in Plasma and Urine at Picogram Level
The method now described was developed to establish the kinetics of idavevine and its metabolite, N-desmethylidaverine, in human plasma in the sub-ng range. The method hinges on a thorough clean-up procedure, which incorporates a solid-liquid extraction of the analytes from plasma, followed by lipid removal by washing with a hydrocarbon solvent. A liquid-liquid extraction is performed to remove acidic plasma constituents. Subsequently NP-HPLC⊗ is performed to separate the analytes from remaining ballast material, with an i.s. pair which functions also as carriers for the analytes. Finally separation and detection are performed by using automated on-column capillary GC with NPD.
KeywordsParent Drug Potassium Carbonate Blank Plasma Determination Limit HPLC Mobile Phase
nitrogenphosphorus detector (N-FID)
internal standard. ‘Ether’ is diethyl ether
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