Micellar Liquid Chromatography
Micelles are notable for their ability to solubilize hydrophobic compounds in aqueous solution. Micellar solutions can help in improving analytical methodologies and in developing entirely new concepts in analytical chemistry; they have been studied especially as HPLC mobile phases. The micelles provide a hydrophobic site for interaction with solutes in the mobile phase and can replace traditional modifiers such as methanol or acetonitrile in RP-HPLC*. We have studied the Chromatographic capabilities of these unique mobile phases — capabilities that are unrealizable with traditional mobile phases. One such advantage is in gradient elution, never popular in repetitive analyses by RP-HPLC because of the slow re-equilibration of the stationary phase after a change in mobile phase composition. We have shown that micelle concentration gradients speed the elution of strongly retained compounds without altering stationary phase ocmposition. This allows a step-gradient back to initial conditions and obviates column re-equilibration. Furthermore, if the conductivity of the two solutions of different micelle concentrations is balanced, micellar gradients are compatible with EC detection — again in contrast with hydro-organic mobile phases. Serum or urine can be directly injected onto a RP column for therapeutic drug monitoring.
KeywordsTherapeutic Drug Monitoring Organic Modifier Lauryl Ether Micellar Liquid Chromatography Mobile Phase Component
critical micelle concentration
quantitative structure-activity relationship
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