Quantitative Determination of Several Prostaglandins and Thromboxane B2 in the Isolated Perfused Rat Brain

  • Claudia Weber
  • Klaus Belsner
  • Barbara Osikowska-Evers
  • Inge Luhmann
  • Michael Höller
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

A GC-MS assay was developed to investigate the time course of the tissue concentrations of PGE2, PGF, PGD2, 6-keto-PGF as well as TXB2 in the isolated rat brain, perfused with a synthetic fluoro-carbon perfusion medium. After perfusion, the brains were frozen in isopentane at −56°, freeze-dried at −40° and pulverized (liq. N2). The powder (~100 mg) was spiked with 4 deuterated analytes and extracted with methanol/water. The supernatants were applied to a BondElut RP-18 cartridge. The eluate was dried down, the residue dissolved in ether/p.e.⌽ and the solution applied to a silica gel column. The eluate was dried down and the residue subjected to methoximation, PFB esterification and silylation. The derivatized COP’s were separated and quantitated by NCI GC-MS.

Keywords

Perfusion Medium Ischaemic Brain Absolute Detection Limit Microwave Fixation Ischaemic Brain Damage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

‘ether’

diethyl

p.e.

petroleum ether (light petroleum)

COP

cyclooxygenase product

PFB

pentafluorobenzyl

NCI

negative-ion chemical ionization

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Claudia Weber
    • 1
  • Klaus Belsner
    • 1
  • Barbara Osikowska-Evers
    • 1
  • Inge Luhmann
    • 1
  • Michael Höller
    • 1
  1. 1.Department of Experimental MedicineJanssen Research FoundationNeuss 21W. Germany

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