Abstract
A GC-MS assay was developed to investigate the time course of the tissue concentrations of PGE2, PGF2α, PGD2, 6-keto-PGF1α as well as TXB2 in the isolated rat brain, perfused with a synthetic fluoro-carbon perfusion medium. After perfusion, the brains were frozen in isopentane at −56°, freeze-dried at −40° and pulverized (liq. N2). The powder (~100 mg) was spiked with 4 deuterated analytes and extracted with methanol/water. The supernatants were applied to a BondElut RP-18 cartridge. The eluate was dried down, the residue dissolved in ether/p.e.⌽ and the solution applied to a silica gel column. The eluate was dried down and the residue subjected to methoximation, PFB esterification and silylation. The derivatized COP’s were separated and quantitated by NCI GC-MS.
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Abbreviations
- ‘ether’:
-
diethyl
- p.e.:
-
petroleum ether (light petroleum)
- COP:
-
cyclooxygenase product
- PFB:
-
pentafluorobenzyl
- NCI:
-
negative-ion chemical ionization
References
Siesjö, B.K. (1981) J. Cerebr. Blood Flow Metab. 1, 155–185.
Wolfe, L.S. (1982) J. Neurochem. 38, 1–14.
Schrör, K. (1986) in Pharmacology of Cerebral Ischaemia (Krieglstein, J., ed.), Elsevier, Amsterdam, pp. 199–209.
Höller, M., Breuer, H. & Fleischhauer, K. (1983) J. Pharmacol. Meths. 9, 170–182.
Mayer, B., Moser, R., Leis, H.J. & Gleisbach, H. (1986) J. Chromatog. 378, 430–436.
Fischer, C. & Meese, C.O. (1985) Biomed. Mass Spectrom. 12, 399–404.
Narumiya, S., Ogoeochi, T., Nakao, K. & Hayaishi, O. (1982) Life Sci. 31, 2093–2103.
Hiroshima, O., Hayashi, H., Ito, S. & Hayaishi, O. (1986) Prostaglandins 32, 63–80.
Wolfe, L.S., Pappius, H.M. & Marion, J. (1976) in Advances in Prostaglandin and Thvomboxane Research, Vol. 1 (Samuelsson, B. & Paoletti, R., eds.), Raven Press, New York, pp. 345–355.
Petroni, A., Socini, A., Blasevich, M., Borghi, A. & Galli, C. (1985) Pvostaglandins 29, 579–587.
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Weber, C., Belsner, K., Osikowska-Evers, B., Luhmann, I., Höller, M. (1988). Quantitative Determination of Several Prostaglandins and Thromboxane B2 in the Isolated Perfused Rat Brain. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_3
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DOI: https://doi.org/10.1007/978-1-4757-9424-3_3
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