The Routine Use, and Column-Stability Implications, of Several Column-Switching HPLC Methods for Determining Drugs in Plasma and Urine

  • J. B. Lecaillon
  • C. Souppart
  • J. P. Dubois
  • A. Delacroix
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


Several possible configurations can be used for column-switching systems:
  • trace enrichment on a pre-column and automatic back-flush or forward-flush injection of the purified sample on an analytical column;

  • injection of the sample onto one column and automatic selection of a portion of the eluate (heart-cutting) for chromatography on a second column;

  • combination of these two systems: trace enrichment and heart-cutting. These approaches are briefly described for metoprolol, CGP 6140 and oxiracetam.


Analytical Column Spiked Sample Metoprolol Tartrate Switching Method Trace Enrichment 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. B. Lecaillon
    • 1
  • C. Souppart
    • 1
  • J. P. Dubois
    • 1
  • A. Delacroix
    • 2
  1. 1.Biopharmaceutical Research CenterLaboratoires CIBA-GEIGYReuil-MalmaisonFrance
  2. 2.Laboratoire de Chimie IndustrielleConservatoire National des Arts et MétiersParisFrance

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