Advertisement

The Analysis of ACE Inhibitors by Immunoassay

  • J. D. Robinson
  • S. Lewis
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

IA* is one of the many techniques that have been used for the analysis of ACE inhibitors in biological fluids. The variety of IA procedures that can be employed are illustrated by the enzyme assays and RIA’s for captopril, cilazapril, enalapril, ramipril and trandolapril. Nearly all of these compounds have been analyzed using different variations of the IA approach. In many cases the assays have been developed to measure concentrations of the active metabolite only, concentrations of the parent compound being determined following in vitro conversion to the metabolite. This article reviews the different IA approaches that have been adopted for analyzing ACE inhibitors and comments on their features of interest.

Keywords

Parent Compound Lysine Analogue Pool Normal Plasma Lysine Derivative Succinimidyl Propionate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

Ab

antibody

ACE

angiotensin converting enzyme

(E)IA

(enzyme) immunoassay

ELISA

enzyme-linked immunosorbent assay

BSA/HSA

bovine/human serum albumin

MCC

4-(maleimidoethyl)cyclohexane carboxylic acid

RIA

radioimmunoassay

References

  1. 1.
    Kinoshita, H., Nakamaru, R., Tanaka, S., Tohira, Y. & Sawada, M. (1986) J. Pharm. Sci. 75, 711–713.CrossRefGoogle Scholar
  2. 2.
    Tanaka, H., Yoneyama, Y., Sugawara, M., Limeda, I. & Ohta, Y. (1987) J. Pharm. Sci. 76, 224–227.CrossRefGoogle Scholar
  3. 3.
    Hichens, M., Hand, E.L. & Mulcahy, W.S. (1981) Ligand Quarterly 4, 43.Google Scholar
  4. 4.
    Worland, P.J. & Jarrott, B. (1986) J. Pharm. Sci. 75, 512–516.CrossRefGoogle Scholar
  5. 5.
    Cordes, E.H., Bull, H.G. & Thornberry, H.G. (1984) in Hypertension and the angiotensin system: Therapeutic approaches (Doyle, A.E. & Beam, A.G., eds.), Raven Press, New York, p. 167.Google Scholar
  6. 6.
    Eckert, H.G., Munscher, G., Oekonomopoulos, R., Urbach, H. & Wissmann, H. (1985) Drug Res. 35, 1251–1256.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. D. Robinson
    • 1
  • S. Lewis
    • 1
  1. 1.Bioanalytical DepartmentHoechst (UK) Ltd.Walton Milton Keynes, BucksUK

Personalised recommendations