Analysis of Angiotensin-Converting Enzyme Inhibitors in Biological Fluids

  • A. Rakhit
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)


During the last 10 years of development of several ACE* inhibitors, diverse analytical methods including HPLC, GC, GC-MS, REI and RIA have been reported. The different analytical methods are reviewed here with respect to their principles, procedures, strengths and weaknesses, except for RIA (considered in the following article). HPLC detection approaches, some entailing appropriate pre-derivatization, include fluorescence, electrochemical and even UV. Whatever the approach, captopril is detectable down to ~10–20 ng/ml, and pentopril to ~50 ng/ml. For GC measurement the approach, depending on the analyze, may be ECD, FID or NPD. Initial reaction steps followed by GC-MS with SIM have also been used for captopril and its mixed disulphides and S-methyl metabolites in different biological fluids. The sensitivity limit ranges between 1 and 20 ng/ml depending on the clean-up method and the detector used.


Hippuric Acid Blank Plasma Prodrug Ester Mixed Disulphide Reactive Thiol Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



angiotensin-converting enzyme


electron-capture detection






mass spectrometry


selected ion monitoring


radioenzymatic inhibition




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  1. 1.
    Ivashkiv, E. (1984) J. Pharm. Sci. 73, 1427–1430.CrossRefGoogle Scholar
  2. 2.
    Migdalof, B.H., Singhvi, S.M. & Kripalini, K.J. (1980) J. Liq. Chromatog. 3, 857–865.CrossRefGoogle Scholar
  3. 3.
    Hayashi, K., Miyamoto, M. & Kripalini, K.J. (1980) J. Chromatog. 338, 161–169.Google Scholar
  4. 4.
    Jarrott, B., Anderson, A., Hooper, R. & Louis, W.J. (1981) J. Pharm. Sci. 70, 665–667.CrossRefGoogle Scholar
  5. 5.
    Toyo’Oka, T., Imai, K. & Kawahara, Y. (1984) J. Pharm. Biomed. Anal. 2, 473–479.CrossRefGoogle Scholar
  6. 6.
    Perrett, D. & Drury, P.L. (1982) J. Liq. Chromatog. 5, 97–110.CrossRefGoogle Scholar
  7. 7.
    Perrett, D., Rudge, S.R. & Drury, P.L. (1984) Bioehem. Soc. Trans. 12, 1059–1060.Google Scholar
  8. 8.
    Shimada, K., Tanaka, M., Nambara, T., Imai, Y., Abe, K. & Yoshinaga, K. (1982) J. Chromatog. 227, 445–451.Google Scholar
  9. 9.
    Bathala, M.S., Weinstein, S.H., Meeker, F.S., Jr., Singhvi, S.M. & Migdalof, B.H. (1984) J. Pharm. Sci. 73, 340–344.CrossRefGoogle Scholar
  10. 10.
    Jemal, M., Ivashkiv, E., Ribick, M. & Cohen, A.I. (1985) J. Chromatog. 345, 299–307.Google Scholar
  11. 11.
    Cohen, A.I., Devlin, R.G., Ivashkiv, E., Funke, P.T. & McCormick, T. (1982) J. Pharm. Sci. 71, 1251–1256.CrossRefGoogle Scholar
  12. 12.
    Ivashkiv, E., McKinstry, D.N. & Cohen, A.I. (1984) J. Pharm. Sci. 73, 1113–1117.CrossRefGoogle Scholar
  13. 13.
    Cohen, A.I., Ivashkiv, E., McCormick, T. & McKinstry, D.N. (1984) J. Pharm. Sci. 73, 1493–1495.CrossRefGoogle Scholar
  14. 14.
    Jemal, M., Ivashkiv, E. & Cohen, A.I. (1985) Biomed. Mass Speetrom. 12, 664–667.CrossRefGoogle Scholar
  15. 15.
    Drummer, O.H., Jarrott, B. & Lousi, W.J. (1984) J. Chromatog. 305, 83–93.Google Scholar
  16. 16.
    Petty, M.A., Reid, J.L. & Miller, S.H.K. (1980) Life Sci. 26, 2045–2050.CrossRefGoogle Scholar
  17. 17.
    Fyrquist, F., Tikkanen, I., Gronhagen-Riska, C, Hortling, L. & Hichens, M. (1984) Clin. Chem. 30, 696–700.Google Scholar
  18. 18.
    Gronhagen-Riska, C., Tikkanen, I. & Fyhrquist, F. (1987) Clin. Chem. 162, 53–60.CrossRefGoogle Scholar
  19. 19.
    Duncan, F.M., Martin, V.I., Williams, B.C., Al-Dujaili, E.A.S. & Edwards, C.R.W. (1983) Clin. Chim. Acta 131, 295–303.CrossRefGoogle Scholar
  20. 20.
    Rakhit, A. & Tipnis, V. (1984) Clin. Chem. 30, 1237–1239.Google Scholar
  21. 21.
    Tipnis, V. & Rakhit, A. (1985) J. Chromatog. 345, 396–401.Google Scholar
  22. 22.
    Swanson, B.N., Stauber, K.L., Alpaugh, W.C. & Weinstein, S.H. (1985) Anal. Bioehem. 148, 401–407.CrossRefGoogle Scholar
  23. 23.
    Reydel-Bax, P., Redalieu, E. & Rakhit, A. (1987) Clin. Chem. 33, 549–553.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. Rakhit
    • 1
  1. 1.Clinical Biology, Research Department Pharmaceuticals DivisionCIBA-GEIGY Corp.SummitUSA

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