Analysis of Angiotensin-Converting Enzyme Inhibitors in Biological Fluids

  • A. Rakhit
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

During the last 10 years of development of several ACE* inhibitors, diverse analytical methods including HPLC, GC, GC-MS, REI and RIA have been reported. The different analytical methods are reviewed here with respect to their principles, procedures, strengths and weaknesses, except for RIA (considered in the following article). HPLC detection approaches, some entailing appropriate pre-derivatization, include fluorescence, electrochemical and even UV. Whatever the approach, captopril is detectable down to ~10–20 ng/ml, and pentopril to ~50 ng/ml. For GC measurement the approach, depending on the analyze, may be ECD, FID or NPD. Initial reaction steps followed by GC-MS with SIM have also been used for captopril and its mixed disulphides and S-methyl metabolites in different biological fluids. The sensitivity limit ranges between 1 and 20 ng/ml depending on the clean-up method and the detector used.

Keywords

Hippuric Acid Blank Plasma Prodrug Ester Mixed Disulphide Reactive Thiol Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

ACE

angiotensin-converting enzyme

ECD

electron-capture detection

FID

flame-ionization

NPD

nitrogen-phosphorus

MS

mass spectrometry

SIM

selected ion monitoring

REI

radioenzymatic inhibition

RIA

radioimmunoassay

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. Rakhit
    • 1
  1. 1.Clinical Biology, Research Department Pharmaceuticals DivisionCIBA-GEIGY Corp.SummitUSA

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