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Enantiomeric Analysis of 2-Phenylpropionic Acid NSAID’s in Biological Fluids by HPLC

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Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 18 A))

Abstract

The importance of stereochemistry in the disposition of the 2-⌽-propionic acid NSAID’s* is outlined. General approaches for the Chromatographic resolution of these agents by both direct and indirect methods are briefly reviewed. Problems associated with the choice of HPLC mobile phase for the resolution of, and derivatization methods for the formation of, stable diastereoisomeric derivatives are illustrated with reference to pirprofen. The application of chiral mobile phases and commercially available HPLC CSP’s to the analysis of these agents is examined, and problems which may arise during the ‘work-up’ of samples of biological origin are discussed.

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Abbreviations

NSAID:

non-steroidal antirinflammatory drug

THF:

tetrahydrofuran

DMOA:

N,N-dimethyloctylamine

CSP:

chiral stationary phase

RP:

reversed phase

MS:

mass spectrometry (EI, electron impact)

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Author information

Authors and Affiliations

  1. Department of Pharmacy, Brighton Polytechnic, Brighton, BN2 4GJ, UK

    A. J. Hutt

  2. Department of Pharmacology and Toxicology, St. Mary’s Hospital Medical School, London, W2 1PG, UK

    J. Caldwell

Authors
  1. A. J. Hutt
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  2. J. Caldwell
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, Guildford, UK

    Eric Reid

  2. Hoechst UK, Milton Keynes, UK

    J. D. Robinson

  3. ICI Pharmaceuticals Division, Macclesfield, UK

    Ian D. Wilson

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© 1988 Springer Science+Business Media New York

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Hutt, A.J., Caldwell, J. (1988). Enantiomeric Analysis of 2-Phenylpropionic Acid NSAID’s in Biological Fluids by HPLC. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_14

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  • DOI: https://doi.org/10.1007/978-1-4757-9424-3_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9426-7

  • Online ISBN: 978-1-4757-9424-3

  • eBook Packages: Springer Book Archive

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