The Use of HPLC in Studies of the Stereoselective Metabolism of Prostaglandin D2

  • Clive Robinson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

PGD2* may be an important mediator of inflammatory reactions. Because of its short biological half-life and its tendency to isomerize or dehydrate, its measurement in complex biological fluids entails problems. To circumvent these, attention has been turned to metabolites of PGD2 that may have longer half-lives and be less susceptible to dehydration. Metabolism in primates including man was known to proceed via the formation of PGF-ring compounds, and later it was verified for urine that the ring hydroxyls have the 9α, 11β-orientation. We have reached similar conclusions for plasma, in volunteers who received 3H-PGD2 by i.v. infusion or by inhalation. Enzymic 11-ketoreduction gives 9α,11β-PGF, which is further metabolized by PGdh. Consideration is given to the identification of these metabolites,-their sites of formation, to analytical implications, and especially to the actual methods used, notably HPLC, GC after derivatization, and MS.

Keywords

Animal Organ Ring Hydroxyl Cytosol Preparation Stereoselective Metabolism Metabolite Identity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

PG

prostaglandin

15-PGdh

15-hydroxyprostaglandin dehydrogenase (EC 1.1.1.41)

3H-

tritiated

MoMeTMS

0-methyloxime, methyl ester, trimethylsilyl ether

MS

mass spectrometry

SIM

selected ion monitoring

LSC

liquid scintillation counting; (relative)retention time, (rel.) t r

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Clive Robinson
    • 1
  1. 1.Department of Clinical PharmacologyUniversity of Southampton, Southampton General HospitalSouthamptonUK

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