The Use of HPLC in Studies of the Stereoselective Metabolism of Prostaglandin D2
PGD2* may be an important mediator of inflammatory reactions. Because of its short biological half-life and its tendency to isomerize or dehydrate, its measurement in complex biological fluids entails problems. To circumvent these, attention has been turned to metabolites of PGD2 that may have longer half-lives and be less susceptible to dehydration. Metabolism in primates including man was known to proceed via the formation of PGF-ring compounds, and later it was verified for urine that the ring hydroxyls have the 9α, 11β-orientation. We have reached similar conclusions for plasma, in volunteers who received 3H-PGD2 by i.v. infusion or by inhalation. Enzymic 11-ketoreduction gives 9α,11β-PGF2α, which is further metabolized by PGdh. Consideration is given to the identification of these metabolites,-their sites of formation, to analytical implications, and especially to the actual methods used, notably HPLC, GC after derivatization, and MS.
KeywordsAnimal Organ Ring Hydroxyl Cytosol Preparation Stereoselective Metabolism Metabolite Identity
15-hydroxyprostaglandin dehydrogenase (EC 188.8.131.52)
0-methyloxime, methyl ester, trimethylsilyl ether
selected ion monitoring
liquid scintillation counting; (relative)retention time, (rel.) t r
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