The Muropeptide Composition of the Peptidoglycan of Staphylococcus aureus Determined with Reversed-Phase High Performance Liquid Chromatography
While extensive early studies on the chemical composition of the cell walls of Staphylococcus aureus have been described in the literature (e.g., Tipper and Strominger, 1965), the high resolution technique of reversed-phase high performance liquid chromatography (HPLC), recently developed by Glauner et al. (1988), has not been applied to staphylococcal cell walls until now. In this communication, we report the results of studies in which the HPLC method, adapted to staphylococci, was used to determine the muropeptide composition of a highly methicillin resistant isolate of S. aureus and some of its transposon mutants with decreased antibiotic resistance.
KeywordsHigh Performance Liquid Chromatography High Performance Liquid Chromatography High Performance Liquid Chromatography Method Beta Lactam Antibiotic Methicillin Resistance
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