Modular Design of the Bi(Multi?)Functional Penicillin-Binding Proteins
The penicilloyl serine transferases are proteins or protein domains that catalyse rupture of the β-lactam amide bond of penicillin and transfer of the penicilloyl moiety to an essential serine. The serine-ester-linked penicilloyl derivatives formed by reaction with the β-lactamases are hydrolytically labile. Those formed by reaction with the penicillin-binding proteins (PBPs) are hydrolytically inert. Penicilloylation of the essential serine of some PBPs produces a signal that is transmitted to the cytosol. Penicilloylation of other PBPs causes loss of a catalytic function related to wall peptidoglycan synthesis. For a recent review and list of references, see Ghuysen (1991).
KeywordsTerminal Domain Neisseria Gonorrhoeae Neisseria Meningitidis Diamino Acid Hydrophobic Cluster Analysis
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- Adam, M., Damblon, C, Jamin, M., Zorzi, W., Dusart, V., Galleni, M., El Kharroubi, A., Piras, G., Spratt, B.G., Keck, W., Coyette, J., Ghuysen, J.M., Nguyen-Distèche, M. and Frère, J.M. (1991) Acyltransferase activities of the high-molecular-mass essential penicillin-binding proteins. Biochem. J. 279, 601–604.PubMedGoogle Scholar
- Granier, B., Duez, C, Lepage, S., Englebert, S., Dusart, J., Dideberg, O., Van Beeumen, J., Frère, J.M. and Ghuysen, J.M. (1992) Primary and predicted secondary structures of the Actinomadura R39 extracellular DD-peptidase, a penicillin-binding protein (PBP) related to the Escherichia coli PBP4. Biochem. J. 228, 781–788.Google Scholar
- Joris, B., Ledent, P., Dideberg, O., Fonzé, E., Lamotte-Brasseur, J., Kelly, J.A., Ghuysen, J.M. and Frère, J.M. (1991) Comparison of the sequences of class A β-lactamases and of the secondary structure elements of penicillin-recognizing proteins. Antimicrob. Ag. Chemother. 35, 2294–2301.CrossRefGoogle Scholar
- Martin, C, Briese, T. and Hakenbeck, R. (1992) Nucleotide sequence of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1A and IB. J. Bacteriol., in press.Google Scholar