A Far Upstream Region is Required for Expression of the ftsI Gene Coding for Penicillin-Binding Protein 3 of Escherichia coli

  • Hiroshi Hara
  • James T. Park
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 65)

Abstract

The ftsI (pbpB) gene of Escherichia coli codes for penicillin-binding protein 3 (PBP 3), a cytoplasmic membrane protein that functions as DD-transpeptidase for the formation of a septum of the murein sacculus and is the principal lethal target of β-lactam antibiotics. PBP 3 was shown to be essential for cell division by analyzing thermosensitiv ftsI mutants and their revertants (Spratt, 1977). The ftsI gene is located at 2 min on the E. coli genetic map, a little distal to leu, and is among a large cluster of murein biosynthetic genes (mur genes and ddl) and cell division genes (fts genes and envA). The DNA sequence of 28 kilobases (kb) of this region including the whole cluster has been determined (EMBL database, entry name EC2MIN).

Keywords

Null Allele Multicopy Plasmid High Copy Number Plasmid Cell Division Gene Cytoplasmic Membrane Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dai, K. and Lutkenhaus, J. (1991) ftsZ is an essential cell division gene in Escherichia coli. J. Bacteriol. 173, 3500–3506.PubMedGoogle Scholar
  2. Hara, H., Yamamoto, Y., Higashitani, A., Suzuki, H. and Nishimura, Y. (1991) Cloning, mapping, and characterization of the Escherichia coliprc gene, which is involved in C-terminal processing of penicillin-binding protein 3. J. Bacteriol. 173,4799–4813.PubMedGoogle Scholar
  3. Ishino, F., Jung, H. K., Dceda, M, Doi, M, Wachi, M. and Matsuhashi, M. (1989) New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division. J. Bacteriol. 171, 5523–5530.PubMedGoogle Scholar
  4. Leclerc, G., Noel, G. and Drapeau, G.R. (1990) Molecular cloning, nucleotide sequence, and expression of shU a new gene in the 2-minute region of the genetic map of Escherichia coli. J. Bacteriol. 172,4696–4700.PubMedGoogle Scholar
  5. Nakamura, ML, Maruyama, I. N., Soma, M., Kato, J., Suzuki, H. and Hirota, Y. (1983) On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3. Mol. Gen. Genet. 191, 1–9.PubMedCrossRefGoogle Scholar
  6. Spratt, B. G. (1977) Temperature-sensitive cell division mutants of Escherichia coli with thermolabile penicillin-binding proteins. J. Bacteriol. 131, 293–305.PubMedGoogle Scholar
  7. Stadler, J. and Adelberg, E. A. (1972) Temperature dependence of sex-factor maintenance in Escherichia coli K-12. J. Bacteriol. 109, 447–449.PubMedGoogle Scholar
  8. Yamaguchi, K. and Tomizawa, J. (1980) Establishment of Escherichia coli cells with an integrated high copy number plasmid. Mol. Gen. Genet 178, 525–533.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Hiroshi Hara
    • 1
  • James T. Park
    • 1
  1. 1.Department of Molecular Biology and MicrobiologyTufts University School of MedicineBostonUSA

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