A Far Upstream Region is Required for Expression of the ftsI Gene Coding for Penicillin-Binding Protein 3 of Escherichia coli
The ftsI (pbpB) gene of Escherichia coli codes for penicillin-binding protein 3 (PBP 3), a cytoplasmic membrane protein that functions as DD-transpeptidase for the formation of a septum of the murein sacculus and is the principal lethal target of β-lactam antibiotics. PBP 3 was shown to be essential for cell division by analyzing thermosensitiv ftsI mutants and their revertants (Spratt, 1977). The ftsI gene is located at 2 min on the E. coli genetic map, a little distal to leu, and is among a large cluster of murein biosynthetic genes (mur genes and ddl) and cell division genes (fts genes and envA). The DNA sequence of 28 kilobases (kb) of this region including the whole cluster has been determined (EMBL database, entry name EC2MIN).
KeywordsNull Allele Multicopy Plasmid High Copy Number Plasmid Cell Division Gene Cytoplasmic Membrane Protein
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