Abstract
The main cytoplasmic precursors of peptidoglycan synthesis are a series of uridine nucleotides which have been previously characterized in Escherichia coli as UDP-N-acetylglucosamine (UDP-GlcNAc), UDP-GlcNAc-enolpyruvate, UDP-N-acetylmuramic acid (UDP-MurNAc) and its four peptide derivatives from UDP-MurNAc-L-Ala to UDP-MurNAc-L-Ala-ǒ-D-Glu-meso-DAP-D-Ala-D-Ala. Their sequential formation is catalyzed by a set of eight highly specific enzymes which were earlier studied in detail (Rogers et al., 1980; Mengin-Lecreulx and van Heijenoort, 1985).
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References
Anwar, R.A. and Vlaovic, M. (1978) Purification of UDP-Wacetylenolpyruvoylglucosamine reductase from Escherichia coli by affinity chromatography, its subunit structure and the absence of flavin as the prosthetic group. Can. J. Biochem. 57, 188–196.
Brosius, J., Dull, T.J., Sleeter, D.D. and Noller, H.F. (1981) Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148, 107–127.
Elledge, S.J. and Walker, G.C. (1985) Phasmid vectors for identification of genes by comlementation of Escherichia coli mutants. J. Bacteriol. 162, 777–783.
Fletcher, G., Irwin, C.A., Henson, J.M., Fillingim, C., Malone, M. and Walker, J.R. (1978) Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-jnurftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J. Bacteriol. 133, 91–100.
Heller, K. and Kadner, R.J. (1985) Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J. Bacteriol. 161, 904–908.
Howard, P.K., Shaw, J. and Otsuka, A.J. (1985) Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli. Gene 35, 321–331.
Ikeda, M., Wachi, M.,. Jung, H.K., Ishino, F. and Matsuhashi, M. (1991) The Escherichia coli mraY gene encoding UDP-N-acetylmuramoy1-pentapeptide: undecapreny 1-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase. J. Bacteriol. 173, 1021–1026.
Kohara, Y., Akiyama, K. and Isono, K. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50, 495–508.
Kroger, M., Wahl, R. and Rice, P. (1991) Compilation of DNA sequences of Escherichia coli (update 1991). Nucleic Acids Res. 19 Supplement, 2023–2043.
Lugtenberg, E.J.J., Wijsman, H.J.W. and van Zaane, D. (1973) Properties of a D-glutamic acid-requiring mutant of Escherichia coli. J. Bacteriol. 114, 499–506.
Medigue, C., Bouché, J.P., Henaut, A. and Danchin, A. (1990) Mapping of sequenced genes (700 kbp) in the restriction map of the Escherichia coli chromosome. Mol. Microbiol. 4, 169–187.
Mengin-Lecreulx, D. and van Heijenoort, J. (1985) Effect of growth conditions on peptidoglycan content and cytoplasmic steps of its biosynthesis in Escherichia coli. J. Bacteriol. 163, 208–212.
Mengin-Lecreulx, D., Parquet, C., Desviat, L.R., Pia, J., Flouret, B., Ayala, J. and van Heijenoort, J. (1989) Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic acid-adding enzyme. J. Bacteriol. 171, 6126–6134.
Mengin-Lecreulx, D., Texier, L., Rousseau, M. and van Heijenoort, J. (1991) The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl(pentapeptide) pyrophosphoryl-undecaprenol N-acetyl-glucosamine transferase involved in the membrane steps of peptidoglycan synthesis. J. Bacteriol. 173, 4625–4636.
Miyakawa, T., Matsuzawa, H., Matsuhashi, M. and Sugino, Y. (1972) Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb. for cell wall peptidoglycan biosynthesis. J. Bacteriol. 112, 950–958.
Pucci, M.J., Discotto, L.F. and Dougherty, T.J. (1992) Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase. J. Bacteriol. 174, 1690–1693.
Rogers, H.J., Perkins, H.R. and Ward, J.B. (1980) Biosynthesis of peptidoglycan, in “Microbial cell walls and membranes”, pp. 239–297. Chapman & Hall, Ltd., London.
Song, W.J. and Jackowski, S. (1992) coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map. J. Bacteriol. 174, 1705–1706.
Venkateswaran, P.S. and Wu, U.C. (1972) Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J. Bacteriol. 110, 935–944.
Wijsman, H.J.W. (1972) A genetic map of several mutations affecting the mucopeptide layer of Escherichia coli. Genet. Res. 20, 65–74.
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Doublet, P., van Heijenoort, J., Mengin-Lecreulx*, D. (1993). Identification of the murI and murB Genes Coding for Cytoplasmic Peptidoglycan Synthetases in the 90-Min Region of the E. coli Chromosome. In: de Pedro, M.A., Höltje, JV., Löffelhardt, W. (eds) Bacterial Growth and Lysis. Federation of European Microbiological Societies Symposium Series, vol 65. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9359-8_16
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DOI: https://doi.org/10.1007/978-1-4757-9359-8_16
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