Abstract
An enzymic flux of O −2 and H2O2 caused strand breaks in the supercoiled covalently closed circular Col El plasmid. Subnanomolar levels of superoxide dismutase or of catalase prevented this attack on DNA, signifying that both O −2 and H2O2 were required. Benzoate, mannitol or histidine, which do not scavenge O −2 and H2O2, also protected the DNA, suggesting that the proximate attacking species had a reactivity comparable to that of the hydroxyl radical. Replacing EDTA with diethylene triamine pentaacetic acid eliminated this attack. In toto the data suggest a metal-catalyzed interaction between O −2 and H2O2 which generates a potent oxidant, possibly OH·, which can cause DNA strand scission. The biological implications of the production and the enzymic scavenging of the superoxide radical are discussed.
Keywords
- Superoxide Dismutase
- Superoxide Radical
- Xanthine Oxidase
- Ethylene Diamine Tetraacetic Acid
- Ethylene Diamine Tetraacetic Acid
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Brawn, K., Fridovich, I. (1980). Superoxide Radical and Superoxide Dismutases: Threat and Defense. In: Simic, M.G., Karel, M. (eds) Autoxidation in Food and Biological Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9351-2_24
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DOI: https://doi.org/10.1007/978-1-4757-9351-2_24
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