The Development of New Poliovirus Vaccines Based on Molecular Cloning

  • Akio Nomoto
  • Michinori Kohara
  • Shusuke Kuge
  • Shinobu Abe
  • Bert L. Semler
  • Toshihiko Komatsu
  • Mineo Arita
  • Heihachi Itoh
Part of the Applied Virology Research book series (AOTP, volume 1)


Poliovirus, known since 1908 to be the causative agent of poliomyelitis (Paul, 1971), is a human enterovirus of the Picornaviridae. This virus consists of a single-stranded RNA of plus-strand polarity and 60 copies each of four capsid proteins, VP1, VP2, VP3, and VP4; it occurs in three serologically distinct types, i.e., type 1, type 2, and type 3.


Vaccine Strain Marker Test Infectious cDNA Clone Reference Virus Paralytic Poliomyelitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Chin, J. (1984). Rev. Infect. Dis. (Suppl.) 6, 581–585.CrossRefGoogle Scholar
  2. Diamond, D. C., Jameson, B. A., Bonin, J., Kohara, M., Abe, S., Itoh, H., Komatsu, T., Arita, M., Kuge, S., Nomoto, A., Osterhaus, A. D. M. E., Crainic, R., and Wimmer, E. (1985). Science 229, 1090–1093.PubMedCrossRefGoogle Scholar
  3. Emini, E. A., Jameson, B. A., Lewis, A. J., Larsen, G. R., and Wimmer, E. (1982). J. Virol. 43, 997–1005.PubMedGoogle Scholar
  4. Emini, E. A., Jameson, B. A., and Wimmer, E. (1983). Nature (Lond.) 304, 699–703.CrossRefGoogle Scholar
  5. Evans, D. M. A., Dunn, G., Minor, P. D., Schild, G. C., Cann, A. J., Stanway, G., Almond, J. W., Currey, K., and Maizel, J. V. (1985). Nature (Lond.) 314, 548–550.CrossRefGoogle Scholar
  6. Gregg, M. B. (1984). Rev. Infect. Dis. (Suppl.) 6, 577–580.CrossRefGoogle Scholar
  7. Hanecak, R., Semler, B. L., Ariga, H., Anderson, C. W., and Wimmer, E. (1984). Cell 37, 1063–1073.PubMedCrossRefGoogle Scholar
  8. Hogle, J. ML, Chow, M., and Filman, D. J. (1985). Science 229, 1358–1363.PubMedCrossRefGoogle Scholar
  9. Holland, J., Spindler, K., Horodyski, F., Grabau, E., Nichol, S., and VandePol, S. (1982). Science 215, 1577–1585.PubMedCrossRefGoogle Scholar
  10. Kew, O. M., Nottay, B. K., Hatch, M. H., Nakano, J. H., and Obijeski, J. F. (1981). J. Gen. Virol. 56, 337–347.PubMedCrossRefGoogle Scholar
  11. Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., Anderson, C. W., and Wimmer, E. (1981). Nature (Lond.) 291, 547–553.CrossRefGoogle Scholar
  12. Kohara, M., Omata, T., Kameda, A., Semler, B. L., Itoh, H., Wimmer, E., and Nomoto, A. (1985). J. Virol. 53, 786–792.PubMedGoogle Scholar
  13. Kohara, M., Abe, S., Kuge, S., Semler, B. L., Komatsu, T., Arita, M., Itoh, H., and Nomoto, A. (1986). Virology 151, 21–30.PubMedCrossRefGoogle Scholar
  14. Kuge, S., Chisaka, O., Kohara, M., Imura, N., Matsubara, K., and Nomoto, A. (1984). J. Biochem. 96, 305–312.PubMedGoogle Scholar
  15. LaMonica, N., Meriam, C., and Racaniello, V. R. (1986). J. Virol. 57, 515–525.Google Scholar
  16. Melnick, J. L. (1984). Rev. Infect. Dis. (Suppl.) 6, 323–327.CrossRefGoogle Scholar
  17. Minor, P. D. (1982). J. Gen. Virol. 59, 307–317.PubMedCrossRefGoogle Scholar
  18. Nomoto, A., Kitamura, N., Golini, F., and Wimmer, E. (1977). Proc. Natl. Acad. Sci. USA 74, 5345–5349.PubMedCrossRefGoogle Scholar
  19. Nomoto, A., Omata, T., Toyoda, H., Kuge, S., Horie, H., Kataoka, Y., Genba, Y., Nakano, Y., and Imura, N. (1982). Proc. Natl. Acad. Sci. USA 79, 5793–5797.PubMedCrossRefGoogle Scholar
  20. Omata, T., Kohara, M., Sakai, Y., Kameda, A., Imura, N., and Nomoto, A. (1984). Gene 32, 1–10.PubMedCrossRefGoogle Scholar
  21. Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., Wimmer, E., and Nomoto, A. (1986). J. Virol. 58, 348–358.PubMedGoogle Scholar
  22. Paul, J. R. (1971). A History of Poliomyelitis. Yale University Press, New Haven, Connecticut.Google Scholar
  23. Racaniello, V. R., and Baltimore, D. (1981a). Proc. Natl. Acad. Sci. USA 78, 4887–4891.PubMedCrossRefGoogle Scholar
  24. Racaniello, V. R., and Baltimore, D. (1981b). Science 214, 916–919.PubMedCrossRefGoogle Scholar
  25. Rossman, M. G., Arnold, E., Erickson, J. W., Frankenberger, E. A., Griffith, J. P., Hecht, H.-J., Johnson, J., Kamer, G., Luo, M., Mosser, A. G., Rueckert, R. R., Sherry, B., and Vriend, G. (1985). Nature (Lond.) 317, 145–153.CrossRefGoogle Scholar
  26. Rueckert, R. R., and Wimmer, E. (1984). J. Virol. 50, 957–959.PubMedGoogle Scholar
  27. Sabin, A. B., and Boulger, L. R. (1973). J. Biol. Stand. 1, 115–118.CrossRefGoogle Scholar
  28. Salk, J. E. (1960). Lancet 2, 715–723.PubMedCrossRefGoogle Scholar
  29. Sarnow, P., Bernstein, H. D., and Baltimore, D. (1986). Proc. Natl. Acad. Sci. USA 83, 571–575.PubMedCrossRefGoogle Scholar
  30. Semler, B. L., Dorner, A. J., and Wimmer, E. (1984). Nucleic Acids Res. 12, 5123–5141.PubMedCrossRefGoogle Scholar
  31. Semler, B. L., Johnson, V. H., and Tracy, S. (1986). Proc. Natl. Acad. Sci. USA 83, 1777–1781.PubMedCrossRefGoogle Scholar
  32. Stanway, G., Cann, A. J., Hauptmann, R., Hughes, P., Clarke, L. D., Mountford, R. C., Minor, P. D., Schild, G. C., and Almond, J. W. (1983). Nucleic Acids Res. 11, 5629–5643.PubMedCrossRefGoogle Scholar
  33. Stanway, G., Hughes, P. J., Mountford, R. C., Reeve, P., Minor, P. D., Schild, G. C., and Almond, J. W. (1984). Proc. Natl. Acad. Sci. USA 81, 1539–1543.PubMedCrossRefGoogle Scholar
  34. Svitkin, Y. V., Maslova, S. V., and Agol, V. I. (1985). Virology 147, 243–252.PubMedCrossRefGoogle Scholar
  35. Toyoda, H., Kohara, M., Kataoka, Y., Suganuma, T., Omata, T., Imura, N., and Nomoto, A. (1984). J. Mol. Biol. 174, 561–585.PubMedCrossRefGoogle Scholar
  36. Toyoda, H., Nicklin, M. J. H., Murray, M. G., Anderson, C. W., Dunn, J. J., Studier, F. W., and Wimmer, E. (1986). Cell 45, 761–770.PubMedCrossRefGoogle Scholar
  37. van der Werf, S., Dreano, M., Bruneau, P., Kopecka, H., and Girard, M. (1983). Gene 23, 85–93.PubMedCrossRefGoogle Scholar
  38. Wetz, K., and Habermehl, K. O. (1979). J. Gen. Virol. 44, 525–534.PubMedCrossRefGoogle Scholar
  39. WHO Consultative Group on Oral Poliomyelitis Vaccine (Sabin Strains) (1977). WHO, BLG/POLIO/77.1, Annex 5.Google Scholar
  40. Wimmer, E. (1982). Cell 28, 199–201.PubMedCrossRefGoogle Scholar
  41. Yogo, Y., and Wimmer, E. (1972). Proc. Natl. Acad. Sci. USA 69, 1877–1882.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Akio Nomoto
    • 1
  • Michinori Kohara
    • 1
    • 2
  • Shusuke Kuge
    • 1
  • Shinobu Abe
    • 2
  • Bert L. Semler
    • 3
  • Toshihiko Komatsu
    • 4
  • Mineo Arita
    • 4
  • Heihachi Itoh
    • 2
  1. 1.Department of Microbiology, Faculty of MedicineUniversity of TokyoHongo, Bunkyo-ku, Tokyo 113Japan
  2. 2.Japan Poliomyelitis Research InstituteJapan
  3. 3.Department of Microbiology and Molecular Genetics, College of MedicineUniversity of CaliforniaIrvineUSA
  4. 4.Department of EnterovirusesNational Institute of HealthMusashimuryama, TokyoJapan

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