Potency Assay of Inactivated Influenza and Poliovaccine

Applications of Single-Radial Immunodiffusion
  • J. M. Wood
  • G. C. Schild
  • P. D. Minor
  • D. I. Magrath
  • Jennifer Mumford
  • R. G. Webster
Part of the Applied Virology Research book series (AOTP, volume 1)


Influenza virus naturally infects humans, pigs, horses, seals, and many different wild and domestic avian species (Hinshaw and Webster, 1982). Inactivated vaccines against influenza are used in humans and in horses and experimental vaccines have been studied in swine and domestic poultry. Vaccination in humans has been shown to induce serum antibody to influenza hemagglutinin (HA), which is capable of virus neutralization and prevention of infection (Dowdle et al., 1974; Hobson et al., 1979). It is therefore important to measure vaccine HA antigen concentration in order to standardize the dose required for effective immunization. Several years ago, single-radial diffusion (SRD) techniques were developed for in vitro potency testing of human influenza vaccines (Wood et al., 1977). The SRD test was shown to be technically simple, reproducible, and sensitive and produced vaccine potency data that related to antigenicity of vaccines (Wood et al., 1977).


Influenza Virus Avian Influenza Influenza Vaccine Potency Test Domestic Poultry 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. M. Wood
    • 1
  • G. C. Schild
    • 2
  • P. D. Minor
    • 1
  • D. I. Magrath
    • 1
  • Jennifer Mumford
    • 3
  • R. G. Webster
    • 4
  1. 1.Division of VirologyNational Institute for Biological Standards and ControlPotters Bar, HertsEngland
  2. 2.National Institute for Biological Standards and ControlPotters Bar, HertsEngland
  3. 3.Equine Virology UnitAnimal Health TrustKennett, Newmarket, SuffolkEngland
  4. 4.Department of Virology and Molecular BiologySt. Jude Children’s Research HospitalMemphisUSA

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