Vaccination with Synthetic Hepatitis B Virus Peptides

  • A. R. Neurath
  • S. B. H. Kent
  • N. Strick
  • K. Parker
Chapter
Part of the Applied Virology Research book series (AOTP, volume 1)

Abstract

Until recently, it was generally accepted that the protein moiety of the hepatitis B virus (HBV) envelope (env) and of sub viral hepatitis B surface antigen particles (HBsAg) consists of a single 25,000-M r species existing in either nonglycosylated (P25) or glycosy-lated forms (GP29). Higher-molecular-weight components detected by polyacrylamide gel electrophoresis (PAGE) of HBV (HBsAg) were considered to represent artifacts of the technique or contaminants (Koistinen, 1980; Peterson et al., 1984). This conclusion was not compatible with results indicating the presence on HBV and on tubular forms of HBsAg of antigenic determinants not present on P25/GP29 (Alberti et al, 1978; Moodie et al, 1974; Neurath et al, 1976). Cloning and sequencing of HBV DNA has provided evidence that the gene coding for HBV env proteins has the capacity to code for proteins larger than the 226 amino acid chain of P25 (Tiollais et al., 1981) (Fig. 1), designated S protein, the translational product of the S region of the HBV env gene. Stibbe and Gerlich (1983) suggested that the minor HBsAg components GP33 and GP36 have the same sequence as P25 plus 55 additional amino acids at the TV-terminus encoded by the pre-S (pre-S2) region of the env gene. They also reported that these components are probably not important for hepatitis B vaccines.

Keywords

Synthetic Peptide None None Virus Peptide HBsAg Particle Hepatitis Research 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. R. Neurath
    • 1
  • S. B. H. Kent
    • 2
  • N. Strick
    • 1
  • K. Parker
    • 2
  1. 1.The Lindsley F. Kimball Research InstituteThe New York Blood CenterNew YorkUSA
  2. 2.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

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