Aspergillus Niger var. Awamori as a Host for the Expression of Heterologous Genes

  • Randy M. Berka
  • Frank T. Bayliss
  • Peggy Bloebaum
  • Daniel Cullen
  • Nigel S. Dunn-Coleman
  • Katherine H. Kodama
  • Kirk J. Hayenga
  • Ronald A. Hitzeman
  • Michael H. Lamsa
  • Melinda M. Przetak
  • Michael W. Rey
  • Lori J. Wilson
  • Michael Ward
Part of the Industry-University Cooperative Chemistry Program Symposia book series (IUCC)

Abstract

Among the diversity of cellular systems that have been developed for the expression of heterologous gene products, certain species of filamentous fungi possess features which make them exceptionally attractive for this purpose. These include (a) the ability to produce high levels (>25 grams per liter) of secreted protein in submerged culture, (b) a long history of safe use in the production of enzymes, antibiotics, and biochemicals which are used for human consumption, and (c) established fermentation processes which are inexpensive by comparison with animal cell culture processes done on a similar scale. These attributes have prompted several biotechnology companies to explore the use of filamentous fungi as hosts for the expression and secretion of foreign proteins. Some of the heterologous gene products which have been made using fungal expression systems are shown in Table 1. Compared to highly refined expression systems such as Escherichia coli or Saccharomyces cerevisiae, the evolution of filamentous fungi as hosts has barely begun, and many fundamental aspects of cell biology and biochemistry in fungi have not been studied. Fortunately, many of the molecular details and principles which have been elucidated in yeast and in mammalian cell systems appear to be applicable to the study of heterologous gene expression and protein secretion in filamentous fungi as well.

Keywords

Filamentous Fungus Shake Flask Culture Aspergillus Nidulans Multiple Drug Resistance Phenyl Methyl Sulfonyl Fluoride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Randy M. Berka
    • 1
  • Frank T. Bayliss
    • 2
  • Peggy Bloebaum
    • 1
  • Daniel Cullen
    • 1
  • Nigel S. Dunn-Coleman
    • 1
  • Katherine H. Kodama
    • 1
  • Kirk J. Hayenga
    • 1
  • Ronald A. Hitzeman
    • 3
  • Michael H. Lamsa
    • 1
  • Melinda M. Przetak
    • 1
    • 2
  • Michael W. Rey
    • 1
  • Lori J. Wilson
    • 1
  • Michael Ward
    • 1
  1. 1.Genencor International Inc.South San FranciscoUSA
  2. 2.San Francisco State UniversitySan FranciscoUSA
  3. 3.University of CaliforniaBerkeleyUSA

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