An Improved Method for in Vitro Susceptibility Testing of Trichomonas Vaginalis

  • Bruno J. Bromke
  • Merewyn C. Furiga
  • Russell C. Hendershot
  • Michelle McGinn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 390)


In vitro susceptibility testing of Trichomonas vaginalis has been performed by many authors.1–10 Most have adapted the classical broth macrodilution methodology to test tubes or microwell plates. However, Lossick11 has criticized susceptibility testing of T. vaginalis for its failure to predict treatment outcome in patients and for lack of standardized practical procedures. For example, some investigators employed an incubation time period that did not correlate with the growth curve of the protozoan,4,10 had no negative growth control,1–10 did not use a standardized inoculum8,10 and/or required an additional subculturing for determination of Minimum Lethal Concentration (MLC). 2,3,5,6,9,10


Susceptibility Testing Initial Cell Density Predict Treatment Outcome Lipid Supplement TRICHOMONAS VAGINALIS 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Bruno J. Bromke
    • 1
  • Merewyn C. Furiga
    • 1
  • Russell C. Hendershot
    • 1
  • Michelle McGinn
    • 1
  1. 1.Department of Microbiology and ImmunologyPhiladelphia College of Osteopathic MedicinePhiladelphiaUSA

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