Embryology of the Endothelial Network: Is There an Hemangioblastic Anlage ?

  • Françoise Dieterlen-Lièvre
  • Dominique Luton
  • Luc Pardanaud
Part of the NATO ASI Series book series (NSSA, volume 263)


Endothelial cells (EC) play a leading role in the modelling of blood vessels. In the embryo they form the blueprint of the vascular tree, the differentiation of which occurs by regionalization of EC properties and apposition of appropriate wall cells. In the adult animal, the turnover of EC, an extremely slow process, accelerates only in a few physiological situations and during tumor progression: formerly quiescent EC becoming reactivated, start multiplying, rupture their basement membrane and migrate. This process, termed angiogenesis (see Folkman, 1974) has been extensively studied at the cellular and biochemical levels. The interplay of growth factors involved either in maintenance of the quiescent state or in reactivation has been investigated. De novo appearance of EC during ontogeny is less well understood. This process, designated vasculogenesis (Pardanaud et al. 1987; Risau and Lemon, 1988) has been partly circumscribed but the cellular, biochemical and molecular events underlying it are still largely elusive.


Chick Embryo Hemopoietic Stem Cell Hemopoietic Cell Avian Embryo Blood Island 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Beaupain, D., Martin, C., Dieterlen-Lièvre, F. (1979). Are developmental hemoglobin changes related to the origin of stem cells and site of erythropoiesis? Blood 53: 212–225.PubMedGoogle Scholar
  2. Coffin J. D. and Poole T. J. (1988). Embryonic vascular development: immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos. Development 102: 735–748.PubMedGoogle Scholar
  3. Cuadros, M.A., Coltey, P., Nieto, M.C., Martin, C. (1992). Demonstration of a phagocytic cell system belonging to the hemopoietic lineage and originating from the yolk sac in the early avian embryo. Development 115: 157–168.PubMedGoogle Scholar
  4. Dieterlen-Lièvre, F., Pardanaud, L., Bolnet C. and Cormier, F. (1990). Development of the hemopoietic and vascular system studied in the avian embryo. In: “The avian model in developmental biology”, éditions du CNRS: pp.319.Google Scholar
  5. Folkman, J. (1974). Tumour angiogenesis. Adv. Cancer Res. 19: 331–358.PubMedCrossRefGoogle Scholar
  6. Le Douarin N.M. (1969). Particularités du noyau interphasique chez la Caille japonaise (Coturnix coturnix japonica). Utilisation de ces particularités comme “marquage biologique” dans les recherches sur les interactions tissulaires et les migrations cellulaires au cours de l’ontogenèse. Bull. Biol. Fr. Belg. 103: 435–452.PubMedGoogle Scholar
  7. Miller A.M. and Mc Whorter J.E. (1914). Experiments on the development of blood vessels in the area pellucida and embryonic body of the chick. Anat. Rec. 8: 203–227.CrossRefGoogle Scholar
  8. Murray P.D.F. (1932). The development in vitro of blood of the early chick embryo. Strangeways Res. Labor. Cambridge: 497–521.Google Scholar
  9. Noden DM (1989) Embryonic origins and assembly of embryonic blood vessels. Ann. Rev. Pulmon. Dis. 140: 1097–1103Google Scholar
  10. Pardanaud, L. and Dieterlen-Lièvre, F. (1993a). Emergence of endothelial and hemopoietic cells in the avian embryo. Anat. and Embryol. 187: 107–114.Google Scholar
  11. Pardanaud, L. and Dieterlen-Lièvre, F. (1993b). Expression of c-etsl in early chick embryo mesoderm: relationship to the hemangioblastic lineage. Cell Adhesion and Commun.(In press).Google Scholar
  12. Pardanaud, L., Altmann, C., Kitos, P., Dieterlen-Lièvre, F. and Buck, C. (1987). Vasculogenesis in the early quail blastodisc as studied with a monoclonal antibody recognizing endothelial cells. Development 100: 339–349.PubMedGoogle Scholar
  13. Pardanaud, L., Yassine, F. and Dieterlen-Lièvre, F. (1989). Relationship between vasculogenesis, angiogenesis and haemopoiesis during avian ontogeny. Development 105: 473–485.PubMedGoogle Scholar
  14. Péault B., Coltey M. and Le Douarin N.M. (1988). Ontogenic emergence of a quail leukocyte/endothelium cell surface antigen. Cell Diff. 23: 165–174.CrossRefGoogle Scholar
  15. Péault B., Thiery J.P. and Le Douarin N.M. (1983). A surface marker for the hemopoietic and endothelial cell lineage in the quail species defined by a monoclonal antibody. Proc. Natl. Acad. Sci. USA. 80: 2976–2980.PubMedCrossRefGoogle Scholar
  16. Reagan F.P. (1915). Vascularization phenomena in fragments of embryonic bodies completely isolated from yolk-sac blastoderm. Anat. Rec. 9: 329–341.CrossRefGoogle Scholar
  17. Risau, W. and Lemmon, V. (1988). Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis. Dev. Biol. 125: 441–450.PubMedCrossRefGoogle Scholar
  18. Stern, C.D., Fraser, S.E., Keynes R.G. and Primmett, D.R.N. (1988). A cell lineage analysis of segmentation in the chick embryo. Development 104 Suppl.: 231–244.PubMedGoogle Scholar
  19. Vandenbunder, B., Pardanaud, L., Jaffredo, T., Mirabel, M.A. and Stéhelin, D. (1989). Complementary patterns of expression of c-etsl, c-myb and c-myc in the blood-forming system of the chick embryo. Development 106: 265–274.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Françoise Dieterlen-Lièvre
    • 1
  • Dominique Luton
    • 1
  • Luc Pardanaud
    • 1
  1. 1.Institut d’Embryologie cellulaire et moléculaireCNRS et du Collège de FranceNogent s/Marne, cédexFrance

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