Angiogenesis pp 213-221 | Cite as

Angiogenic Polypeptides in Breast Cancer: Expression of Mrna’s in Primary Human Tumours, MCF-7 Cell Transfection and Xenograft Models

  • Hua-Tang Zhang
  • Rangana Choudhuri
  • Prudence AE Scott
  • Lyna Zhang
  • Marina Ziche
  • Lucia Morbidelli
  • Sandra Donnini
  • Rhys T. Jagger
  • Hock-Ye Chan
  • Kenneth Smith
  • Sandra Peak
  • Margaret C. P. Rees
  • Adrian L. Harris
  • Roy Bicknell
Chapter
Part of the NATO ASI Series book series (NSSA, volume 298)

Abstract

Screening of 84 primary human breast carcinomas for the mRNA expression of seven angiogenic polypeptides showed that the most commonly expressed and/or the most highly expressed when compared to normal breast tissue were vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor/thymidine Phosphorylase (PDECGF/TP)1. The neurokines midkine (MK) and pleiotrophin were also fairly commonly expressed in tumour but not normal tissue (unpublished data from this group and ref. 1).

Keywords

Vascular Endothelial Growth Factor Thymidine Phosphorylase Angiogenic Activity Vascular Endothelial Cell Growth Factor Vascular Endothelial Growth Factor Isoforms 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Reif M, LeJeune S, Scott PAE, Fox S, Smith K, Leek R, Moghaddam A, Whitehouse R, Bicknell R, Harris AL. Expression of the angiogenic factors vascular endothelial cell growth factor, acidic and basic fibroblast growth factor, transforming growth factor b-1, platelet-derived endothelial cell growth factor, placental growth factor, and pleiotrophin in human primary breast cancer and its relation to angiogenesis. Cancer Res 57:963–969, 1997Google Scholar
  2. 2.
    O’Brien T, Cranston D, Fuggle S, Bicknell R, Harris AL. The angiogenic factor midkine is expressed in bladder cancer and overexpression correlates with a poor outcome in patients with invasive cancers. Cancer Res 56: 2515–2518, 1996PubMedGoogle Scholar
  3. 3.
    Bicknell R. Mechanistic insights into tumour angiogenesis. in “Tumour Angiogenesis” Ed. Bicknell R, Lewis CE, Ferrara N, pp 19–28, Oxford University Press, 1997Google Scholar
  4. 4.
    Choudhuri R, Zhang H.T., Donnini S, Ziche M, Bicknell R. An angiogenic role for the neurokines midkine and pleiotrophin in tumourigenesis. Cancer Res 57: 1814–1819, 1997PubMedGoogle Scholar
  5. 5.
    Zhang HT, Craft P, Scott PAE, Ziche M, Weich HA, Harris AL, Bicknell R. Enhancement of tumour growth and vascular density by transfection of vascular endothelial cell growth factor into MCF-7 human breast carcinoma cells. J Natl Cancer Inst 87: 213–219, 1995CrossRefPubMedGoogle Scholar
  6. 6.
    Scott PAE. Vascular endothelial growth factor in breast carcinoma. D. Phil thesis, University of Oxford, 1996Google Scholar
  7. 7.
    Gasparini G. Prognostic and predictive value of intra-tumoral microvessel density in human solid tumours. In “Tumour Angiogenesis” Ed. Bicknell R, Lewis CE Ferrara N, pp 29–44. Oxford University Press, 1997Google Scholar
  8. 8.
    Moghaddam A and Bicknell R. Expression of platelet-derived endothelial cell growth factor in E coli and confirmation of its thymidine Phosphorylase activity. Biochemistry, 31: 12141–12146, 1992CrossRefPubMedGoogle Scholar
  9. 9.
    Usuki K, Saras J, Waltenberger J, Miyazono K, Pierce G, Thomason A, Heldin CH. Platelet-derived endothelial cell growth factor has thymidine Phosphorylase activity. Biochem Biophys Res Commun 184: 1311–1316, 1992CrossRefPubMedGoogle Scholar
  10. 10.
    Finnis C., Dodsworth N, Pollitt CE, Carr G and Sleep D. Thymidine Phosphorylase activity of platelet-derived endothelial cell growth factor is responsible for endothelial cell mitogenicity. Eur J Biochem 212: 201–210, 1993CrossRefPubMedGoogle Scholar
  11. 11.
    Griffiths L, Dachs GU, Bicknell R, Harris AL and Stratford IJ. The influence of oxygen tension and pH on the expression of platelet-derived endothelial cell growth factor / thymidine Phosphorylase in human breast tumour cells grown in vitro and in vivo. Cancer Res 57: 570–572, 1997Google Scholar
  12. 12.
    Moghaddam A, Zhang HT, Fan TPD, Hu DE, Lees VC, Turley H, Fox SB, Gatter KC, Harris AL, Bicknell R. Thymidine Phosphorylase is angiogenic and promotes tumour growth. Proc Natl Acad Sci USA 92, 998–1002, 1995CrossRefPubMedGoogle Scholar
  13. 13.
    Ishikawa F, Miyazono K, Hellman U, Drexler H, Wernstedt C., Hagiwara K et al. Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factors. Nature 338: 557–562, 1989CrossRefPubMedGoogle Scholar
  14. 14.
    Moghaddam A, Choudhuri R, Bicknell R. Thymidine phosphorylase/platelet-derived endothelial cell growth factor: an angiogenic factor, in “Tumour Angiogenesis” Ed. Bicknell R, Lewis CE, Ferrara N, pp 251–260, Oxford University Press, 1997Google Scholar
  15. 15.
    Reynolds K, Farzaneh F, Collins WP, Campbell S, Bourne TH, Lawton F, Moghaddam A, Harris AL, Bicknell R. Association of ovarian malignancy with expression of platelet-derived endothelial cell growth factor. J Natl Cancer Inst 86: 1234–1238, 1994CrossRefPubMedGoogle Scholar
  16. 16.
    Fox SB, Westwood M, Moghaddam A, Comley M, Turley H, Whitehouse RM, Bicknell R, Gatter KC, Harris AL. The angiogenic factor platelet-derived endothelial cell growth factor / thymidine Phosphorylase is up-regulated in breast cancer epithelium. Brit J Cancer 73: 275–280, 1996CrossRefPubMedGoogle Scholar
  17. 17.
    Takahashi Y, Bucana CD, Liu W, Yoneda J, Kitadai Y, Cleary KR et al. Platelet-derived endothelial cell growth factor in human colon cancer angiogenesis: role of infiltrating cells. J Natl Cancer Inst 88:1146–1151, 1996CrossRefPubMedGoogle Scholar
  18. 18.
    Takebayashi Y, Akiyama S, Akiba S, Yamada K, Miyadera K, Sumizawa T et al. Clinicopathologic and prognostic significance of an angiogenic factor, thymidine Phosphorylase, in human colorectal cancer. J Natl Cancer Inst 88: 1110–1117, 1996CrossRefPubMedGoogle Scholar
  19. 19.
    Folkman J. What is the role of thymidine Phosphorylase in tumour angiogenesis? J Natl Cancer Inst 88: 1091–1092, 1996CrossRefPubMedGoogle Scholar
  20. 20.
    Haraguchi M, Kazutaka M, Uemura K, Sumizawa T, Furukawa T, Yamada K, Akiyama SI. Angiogenic activity of enzymes. Nature 368: 198, 1994CrossRefPubMedGoogle Scholar
  21. 21.
    Jagger RT, Bicknell R. Vascular targeting of anti-cancer gene therapy, in “Tumour Angiogenesis” Ed: Bicknell R, Lewis CE, Ferrara N, pp 357–376, Oxford University Press, 1997Google Scholar
  22. 22.
    Fox SB, Turner GDH, Gatter KC, Harris AL. The increased expression of adhesion molecules ICAM-3 and E- and P-selectins on breast cancer endothelium. J Pathol 177:369–376, 1995CrossRefPubMedGoogle Scholar
  23. 23.
    Brown LF, Berse B, Jackman RW, Tognazzi K, Manseau EJ, Dvorak HF, Senger DR. Increased expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in kidney and bladder carcinomas. Am J Pathol 143: 1255–1262, 1993PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Hua-Tang Zhang
    • 1
  • Rangana Choudhuri
    • 1
  • Prudence AE Scott
    • 1
  • Lyna Zhang
    • 1
    • 4
  • Marina Ziche
    • 5
  • Lucia Morbidelli
    • 5
  • Sandra Donnini
    • 1
    • 5
  • Rhys T. Jagger
    • 1
  • Hock-Ye Chan
    • 1
  • Kenneth Smith
    • 2
  • Sandra Peak
    • 3
  • Margaret C. P. Rees
    • 4
  • Adrian L. Harris
    • 2
  • Roy Bicknell
    • 1
  1. 1.Molecular Angiogenesis GroupUSA
  2. 2.Growth Factor Group, Imperial Cancer Research Fund, Institute of Molecular Medicine, John Radcliffe HospitalUniversity of OxfordHeadington, OxfordUK
  3. 3.Imperial Cancer Research FundClare Hall LaboratoriesSouth Mimms, HertsUK
  4. 4.Nuffield Department of Obstetrics and Gynaecology, John Radcliffe HospitalUniversity of OxfordOxfordUK
  5. 5.Department of PharmacologyUniversity of FlorenceFlorenceItaly

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