Angiogenesis pp 99-112 | Cite as

Autocrine Role of Basic Fibroblast Growth Factor (bFGF) in Angiogenesis and Angioproliferative Diseases

  • Anna Gualandris
  • Patrizia Dell’Era
  • Marco Rusnati
  • Roberta Giuliani
  • Elena Tanghetti
  • Maria Pia Molinari-Tosatti
  • Marina Ziche
  • Domenico Ribatti
  • Marco Presta
Part of the NATO ASI Series book series (NSSA, volume 298)


bFGF belongs to the family of the heparin-binding growth factors (Basilico and Moscatelli, 1992). The single copy human bFGF gene encodes multiple bFGF isoforms with molecular weights ranging from 24 kD to 18 kD. High molecular weight isoforms (HMW-bFGFs) are colinear NH2-terminal extensions of the better characterized 18 kD protein (Florkiewicz and Sommer, 1989). Both low and high molecular weight bFGFs exert angiogenic activity in vivo and induce cell proliferation, protease production, and Chemotaxis in endothelial cells in vitro (Gualandris, Urbinati, Rusnati, Ziche, and Presta, 1994). Also, bFGF has been shown to stimulate endothelial cells to form capillary-like structures in collagen gels (Montesano, Vassalli, Baird, Guillemin, and Orci, 1989) and to invade the amniotic membrane in vitro (Mignatti, Tauboi, Robbins, and Rifkin, 1989). Moreover, the phenotype induced in vitro by bFGF in endothelial cells includes modulation of integrin expression (Klein, Giancotti, Presta, Albelda, Buck, and Rifkin, 1993), gap-junctional intercellular communication (Pepper and Meda, 1992) and urokinase receptor upregulation (Mignatti, Mazzieri, and Rifkin, 1991a). Studies with neutralizing anti-bFGF antibodies have implicated endogenous bFGF in wound repair (Broadly, Aquino, Woodward, Bickley-Sturrock, Sat, Rifkin, and Davidson, 1989), vascularization of the chorioallantoic membrane during chick embryo development (Ribatti, Urbinati, Nico, Rusnati, Roncali, and Presta, 1995), and tumor growth under defined experimental conditions (Baird, Mormède, and Bohlen, 1986; Gross, Herblin, Dusak, Czerniak, Diamond, Sun, Eidsvoog, Dexter, and Yoayon, 1993).


Basic Fibroblast Growth Factor Acquire Immune Deficiency Syndrome Amniotic Membrane Normal Endothelial Cell bFGF mRNA 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Anna Gualandris
    • 1
  • Patrizia Dell’Era
    • 1
  • Marco Rusnati
    • 1
  • Roberta Giuliani
    • 1
  • Elena Tanghetti
    • 1
  • Maria Pia Molinari-Tosatti
    • 2
  • Marina Ziche
    • 3
  • Domenico Ribatti
    • 4
  • Marco Presta
    • 1
  1. 1.Unit of General Pathology and ImmunologyUniversity of BresciaBresciaItaly
  2. 2.Unit of Histology, Department of Biomedical Sciences and BiotechnologyUniversity of BresciaBresciaItaly
  3. 3.Department of PharmacologyUniversity of FlorenceFlorenceItaly
  4. 4.Institute of Anatomy, Histology and EmbryologyUniversity of BariBariItaly

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