Summary
The nucleophilic properties of human lactoferrrin (Lf) were demonstrated by immunofluorescence microscopy using cryostat rat tissue sections, and the nuclear/perinuclear distribution of Lf in ethanol-fixed human neutrophils was visualized with rabbit anti-human Lf, producing a P-ANCA/GS-ANA staining pattern. Prevention of complement activation by Lf was confirmed in a haemolytic assay. Antibodies (IgG) against human Lf were studied by ELISA in sera from patients with Crohn’s disease, ulcerative colitis, primary sclerosing cholangitis, rheumatoid arthritis, systemic lupus erythematous and primary Sjögren’s syndrome. Anti-Lf antibodies were found in high frequency in ulcerative colitis and primary sclerosing cholangitis, but only occasionally in the other conditions.
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References
Birgens H. The interaction of lactoferrin with human monocytes. (Review) Dan.Med. Bull. 1991; 38: 244252
Briggs RC, Glass II WF, Montiel MM, Hnilica LS (1981): Lactoferrin: Nuclear localizaation in the human neutrophilic granulocyte? J.Histochem.Cytochem. 29: 1128–36
Coremans IEM, Hagen EC, van der Woude FJ et al (1992): Anti-lactoferrin antibodies in patients with rheumatoid arthritis with vasculitis. Clin.Rheumatol. 11: 4
Davidson LA, Lönnerdal B. Specific binding of lactoferrin to brush border menbrane: ontogeny and effect of glycan chain. Am.J.Physiol. 1988; 254: G580–85
Esaguy N, Aguas AP, van Embden JDA, Silva MT (1991): Mycobacteria and human autoimmune disease: direct evidence for cross-reactivity between human lactoferrin and the 65-kilodalton protein of tubercle and leprosy bacilli. Inf.Immun. 59: 1117–25
Goldschmeding R. Target antigens of antineutrophil cytoplasmic antibodies. Thesis (MD), Amsterdam 1992
Kijlstra A. The role of lactoferrin in the nonspecific immune response on the occular surface. Reg.Immunol. 1990–91; 3: 193–97
Peen E, Abner S, Bodemar G, Ryden B-0, Sjölin C, Tejle K, Skogh T (1993): Anti-lactoferrin antibodies and other types of “ANCA” in Crohn’s disease, ulcerative colitis, and primary sclerosing cholangitis. Gut 34: 56–62
Skogh T, Dahlgren C, Holmgren K, Peen E, Stendahl 0 (1991): Anti-granulocyte anti-bodies (C-ANCA, P-ANCA, GS-ANA) studied by confocal scanning laser fluorescence microscopy, ELISA, and chemiluminescence techniques. Scand J Immunol. 34: 137–45
Wakefield AJ, Sankey EA, Dhillon AP, Sawyerr AM, More L, Sim R, Pittilo RM, Rowles PM, Hudson M, Lewis AAM, Pounder RE (1991): Granulomatous vasculitis in Crohn’s disease. Gastroenterology 100: 1279–87
Wiik A (1980): Granulocyte-specific antinuclear antibodies. Allergy 35: 263–89
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© 1993 Springer Science+Business Media New York
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Skogh, T., Peen, E. (1993). Lactoferrin, Anti-Lactoferrin Antibodies and Inflammatory Disease. In: Gross, W.L. (eds) ANCA-Associated Vasculitides. Advances in Experimental Medicine and Biology, vol 336. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9182-2_97
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DOI: https://doi.org/10.1007/978-1-4757-9182-2_97
Publisher Name: Springer, Boston, MA
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